Grund S, Eichberg J, Engel W
Ultramicroscopy. 1979;3(3):293-304. doi: 10.1016/s0304-3991(79)80039-x.
The effect of various preparations on the emission image was studied taking into account the influence of UV wave length, section thickness and specimen support. By suitable selection of these parameters, images with sufficient contrast can be obtained of unstained specimens which are only fixed with glutaraldehyde. With glutaraldehyde fixation intravitally injected Biligrafin used as a stain in cholecystography is recognized in the emission image of liver tissue labeling the intravital transport path from the blood capillaries to the bile ducts. Hypotheses for the image forming process are discussed.
考虑到紫外线波长、切片厚度和标本载体的影响，研究了各种制剂对发射图像的作用。通过对这些参数的适当选择，可以获得仅用戊二醛固定的未染色标本的具有足够对比度的图像。在活体注射用作胆囊造影剂的胆影葡胺进行戊二醛固定后，在肝组织的发射图像中可识别出它，标记了从毛细血管到胆管的活体运输路径。文中讨论了图像形成过程的假设。
