Sewell B T, Bouloukos C, von Holt C
J Microsc. 1984 Oct;136(Pt 1):103-12. doi: 10.1111/j.1365-2818.1984.tb02550.x.
Glutaraldehyde and formaldehyde have been used to fix chromatin core particles for electron microscopy. Glutaraldehyde crosslinks protein only, whereas formaldehyde crosslinks protein and DNA. This is confirmed by the observation that the detergents sodium dodecyl sulphate, Sarkosyl NL 35 and benzylalkyldimethyl ammonium chloride separate the DNA from the protein in the case of glutaraldehyde fixed core particles but not in the case of formaldehyde fixed core particles. The fixative used in the preparation must therefore be considered as a further variable when evaluating electron microscopic images of chromatin.
戊二醛和甲醛已被用于固定染色质核心颗粒以进行电子显微镜观察。戊二醛仅使蛋白质交联，而甲醛使蛋白质和DNA交联。这一点可通过以下观察结果得到证实：对于戊二醛固定的核心颗粒，去污剂十二烷基硫酸钠、 Sarkosyl NL 35和苄基烷基二甲基氯化铵能将DNA与蛋白质分离，而对于甲醛固定的核心颗粒则不能。因此，在评估染色质的电子显微镜图像时，制备过程中使用的固定剂必须被视为另一个可变因素。
