Hwang M G, Chung I K, Kang B G, Cho M H
Department of Biology, Yonsei University, 120-749, Seoul, South Korea.
FEBS Lett. 2001 Aug 10;503(1):35-40. doi: 10.1016/s0014-5793(01)02685-0.
We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a single Myb telomeric DNA binding domain at the C-terminus and an extensive homology with other known telomere-binding proteins. The isolated C-terminus of AtTBP1 is capable of sequence-specific DNA binding to plant duplex telomeric DNA. These results suggest that AtTBP1 may play important roles in plant telomere function in vivo.
我们鉴定出了拟南芥的一个cDNA，命名为AtTBP1，它编码一种预测大小为70.6 kDa的蛋白质，该蛋白质能特异性结合植物端粒重复序列TTTAGGG。AtTBP1在拟南芥基因组中作为单拷贝基因存在，并在各个器官中普遍表达。AtTBP1在C端有一个单一的Myb端粒DNA结合结构域，与其他已知的端粒结合蛋白具有广泛的同源性。分离得到的AtTBP1的C端能够与植物双链端粒DNA进行序列特异性的DNA结合。这些结果表明AtTBP1可能在植物体内端粒功能中发挥重要作用。
