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碳纳米材料与等离子体活化碳纳米材料溶液与端粒结合蛋白的相互作用研究。

Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein.

机构信息

Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 139-701, Korea.

Division of Sustainable Technology, Rudraksh Proudhyogiki Sangathan, Delhi, India.

出版信息

Sci Rep. 2017 Jun 1;7(1):2636. doi: 10.1038/s41598-017-02690-4.

DOI:10.1038/s41598-017-02690-4
PMID:28572671
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5454022/
Abstract

Most cancer cells have telomerase activity because they can express the human telomerase reverse transcriptase (hTERT) gene. Therefore, the inhibition of the hTERT expression can play an important role in controlling cancer cell proliferation. Our current study aims to inhibit hTERT expression. For this, we synthesized graphene oxide (GO) and a functionalized multiwall carbon nanotube (f-MWCNT), latter treated them with cold atmospheric pressure plasma for further analysis of the hTERT expression. The inhibition of hTERT expression by GO, f-MWCNT, plasma activated GO solution (PGOS), and plasma activated f-MWCNT solution (PCNTS), was studied using two lung cancer cell lines, A549 and H460. The hTERT experimental results revealed that GO and PGOS sufficiently decreased the hTERT concentration, while f-MWCNT and PCNTS were unable to inhibit the hTERT concentration. Therefore, to understand the inhibition mechanism of hTERT, we studied the binding properties of GO and PGOS with telomere binding protein (AtTRB2). The interaction studies were carried out using circular dichroism, fluorescence, H-N NMR spectroscopy, and size-exclusion chromatography (SEC) binding assay. We also used docking simulation to have an better understanding of the interactions between GO nanosheets and AtTRB2 protein. Our results may provide new insights that can benefit in biomedical treatments.

摘要

大多数癌细胞都具有端粒酶活性，因为它们可以表达人类端粒酶逆转录酶（hTERT）基因。因此，抑制 hTERT 的表达可以在控制癌细胞增殖方面发挥重要作用。我们目前的研究旨在抑制 hTERT 的表达。为此，我们合成了氧化石墨烯（GO）和功能化多壁碳纳米管（f-MWCNT），并用冷等静压等离子体对其进行处理，以进一步分析 hTERT 的表达。使用两种肺癌细胞系 A549 和 H460 研究了 GO、f-MWCNT、等离子体激活 GO 溶液（PGOS）和等离子体激活 f-MWCNT 溶液（PCNTS）对 hTERT 表达的抑制作用。hTERT 的实验结果表明，GO 和 PGOS 充分降低了 hTERT 的浓度，而 f-MWCNT 和 PCNTS 则不能抑制 hTERT 的浓度。因此，为了了解 hTERT 抑制的机制，我们研究了 GO 和 PGOS 与端粒结合蛋白（AtTRB2）的结合特性。使用圆二色性、荧光、H-NMR 光谱和尺寸排阻色谱（SEC）结合测定法进行了相互作用研究。我们还使用对接模拟来更好地了解 GO 纳米片与 AtTRB2 蛋白之间的相互作用。我们的结果可能提供新的见解，有助于生物医学治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/fcb3ca7ce99f/41598_2017_2690_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/ad0e3ed1c7ed/41598_2017_2690_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/a4c458784a98/41598_2017_2690_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/1ac6e31771c5/41598_2017_2690_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/efaac22691a8/41598_2017_2690_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/0e3f0df9ade6/41598_2017_2690_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/fd114c6e619c/41598_2017_2690_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/c2c0ef501f0d/41598_2017_2690_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/521b68ec86ee/41598_2017_2690_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/fcb3ca7ce99f/41598_2017_2690_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/ad0e3ed1c7ed/41598_2017_2690_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/a4c458784a98/41598_2017_2690_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/1ac6e31771c5/41598_2017_2690_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/efaac22691a8/41598_2017_2690_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/0e3f0df9ade6/41598_2017_2690_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/fd114c6e619c/41598_2017_2690_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/c2c0ef501f0d/41598_2017_2690_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/521b68ec86ee/41598_2017_2690_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f6e/5454022/fcb3ca7ce99f/41598_2017_2690_Fig9_HTML.jpg

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