啤酒腐败菌短乳杆菌中的啤酒花抗性由ATP结合盒多药转运蛋白HorA介导。
Hop resistance in the beer spoilage bacterium Lactobacillus brevis is mediated by the ATP-binding cassette multidrug transporter HorA.
作者信息
Sakamoto K, Margolles A, van Veen H W, Konings W N
机构信息
Brewing Research & Development Laboratory, Asahi Breweries, Ltd., Moriya-machi, Kitasoma-gun, Ibaraki 302-0106, Japan.
出版信息
J Bacteriol. 2001 Sep;183(18):5371-5. doi: 10.1128/JB.183.18.5371-5375.2001.
Abstract
Lactobacillus brevis is a major contaminant of spoiled beer. The organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. The hop resistance in L. brevis is, at least in part, dependent on the expression of the horA gene. The deduced amino acid sequence of HorA is 53% identical to that of LmrA, an ATP-binding cassette multidrug transporter in Lactococcus lactis. To study the role of HorA in hop resistance, HorA was functionally expressed in L. lactis as a hexa-histidine-tagged protein using the nisin-controlled gene expression system. HorA expression increased the resistance of L. lactis to hop compounds and cytotoxic drugs. Drug transport studies with L. lactis cells and membrane vesicles and with proteoliposomes containing purified HorA protein identified HorA as a new member of the ABC family of multidrug transporters.
摘要
短乳杆菌是变质啤酒的主要污染物。尽管啤酒中存在赋予其苦味的抗菌啤酒花化合物，但该微生物仍能在啤酒中生长。短乳杆菌对啤酒花的抗性至少部分取决于horA基因的表达。HorA推导的氨基酸序列与乳酸乳球菌中的一种ATP结合盒式多药转运蛋白LmrA的序列有53%的同一性。为了研究HorA在啤酒花抗性中的作用，利用乳链菌肽控制的基因表达系统，在乳酸乳球菌中功能性表达了带有六组氨酸标签的HorA蛋白。HorA的表达增加了乳酸乳球菌对啤酒花化合物和细胞毒性药物的抗性。对乳酸乳球菌细胞、膜囊泡以及含有纯化HorA蛋白的蛋白脂质体进行的药物转运研究表明，HorA是ABC多药转运蛋白家族的一个新成员。
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本文引用的文献
1
A plasmid pRH45 of Lactobacillus brevis confers hop resistance.短乳杆菌的质粒pRH45赋予啤酒花抗性。
J Gen Appl Microbiol. 1998 Oct;44(5):361-363. doi: 10.2323/jgam.44.361.
2
Molecular properties of bacterial multidrug transporters.细菌多药转运蛋白的分子特性
Microbiol Mol Biol Rev. 2000 Dec;64(4):672-93. doi: 10.1128/MMBR.64.4.672-693.2000.
3
Antibiotic resistance: era of the multidrug pump.
Mol Microbiol. 2000 May;36(3):772-3. doi: 10.1046/j.1365-2958.2000.01871.x.
4
The homodimeric ATP-binding cassette transporter LmrA mediates multidrug transport by an alternating two-site (two-cylinder engine) mechanism.同二聚体ATP结合盒转运蛋白LmrA通过交替双位点（双缸发动机）机制介导多药转运。
EMBO J. 2000 Jun 1;19(11):2503-14. doi: 10.1093/emboj/19.11.2503.
5
The purified and functionally reconstituted multidrug transporter LmrA of Lactococcus lactis mediates the transbilayer movement of specific fluorescent phospholipids.乳酸乳球菌经纯化且功能重建的多药转运蛋白LmrA介导特定荧光磷脂的跨膜运动。
Biochemistry. 1999 Dec 7;38(49):16298-306. doi: 10.1021/bi990855s.
6
Restrictive use of detergents in the functional reconstitution of the secondary multidrug transporter LmrP.在次生多药转运蛋白LmrP的功能重建中限制洗涤剂的使用。
Biochemistry. 1999 Jan 19;38(3):1002-8. doi: 10.1021/bi981863w.
7
The ABC family of multidrug transporters in microorganisms.微生物中的多药转运蛋白ABC家族。
Biochim Biophys Acta. 1998 Jun 10;1365(1-2):31-6. doi: 10.1016/s0005-2728(98)00039-5.
8
A bacterial antibiotic-resistance gene that complements the human multidrug-resistance P-glycoprotein gene.一种可补充人类多药耐药性P-糖蛋白基因的细菌抗生素抗性基因。
Nature. 1998 Jan 15;391(6664):291-5. doi: 10.1038/34669.
9
Extraction of Hoechst 33342 from the cytoplasmic leaflet of the plasma membrane by P-glycoprotein.P-糖蛋白从质膜细胞质小叶中提取Hoechst 33342。
Eur J Biochem. 1997 Nov 15;250(1):122-9. doi: 10.1111/j.1432-1033.1997.00122.x.
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