枯草芽孢杆菌的 CotA 是一种铜依赖性漆酶。
CotA of Bacillus subtilis is a copper-dependent laccase.
作者信息
Hullo M F, Moszer I, Danchin A, Martin-Verstraete I
机构信息
Unité de Génétique des Génomes Bactériens, Institut Pasteur, 75724 Paris Cedex 15, France.
出版信息
J Bacteriol. 2001 Sep;183(18):5426-30. doi: 10.1128/JB.183.18.5426-5430.2001.
Abstract
The spore coat protein CotA of Bacillus subtilis displays similarities with multicopper oxidases, including manganese oxidases and laccases. B. subtilis is able to oxidize manganese, but neither CotA nor other sporulation proteins are involved. We demonstrate that CotA is a laccase. Syringaldazine, a specific substrate of laccases, reacted with wild-type spores but not with DeltacotA spores. CotA may participate in the biosynthesis of the brown spore pigment, which appears to be a melanin-like product and to protect against UV light.
摘要
枯草芽孢杆菌的芽孢衣蛋白CotA与多铜氧化酶具有相似性，包括锰氧化酶和漆酶。枯草芽孢杆菌能够氧化锰，但这一过程不涉及CotA或其他芽孢形成蛋白。我们证明CotA是一种漆酶。漆酶的特异性底物丁香醛连氮与野生型孢子发生反应，但不与缺失CotA基因的孢子反应。CotA可能参与棕色孢子色素的生物合成，该色素似乎是一种类似黑色素的产物，具有抵御紫外线的作用。
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