Venter Marietjie, Madhi Shabir A, Tiemessen Caroline T, Schoub Barry D
National Institute for Virology, Private bag X4, 2131 Sandringham, South Africa1.
Pediatric Infectious Disease Research Unit, University of the Witwatersrand, South Africa2.
J Gen Virol. 2001 Sep;82(Pt 9):2117-2124. doi: 10.1099/0022-1317-82-9-2117.
The molecular epidemiology of respiratory syncytial virus (RSV) was studied over four consecutive seasons (1997-2000) in a single tertiary hospital in South Africa: 225 isolates were subgrouped by RT-PCR and the resulting products sequenced. Subgroup A predominated in two seasons, while A and B co-circulated approximately equally in the other seasons. The nucleotide sequences of the C-terminal of the G-protein were compared to sequences representative of previously defined RSV genotypes. South African subgroup A and subgroup B isolates clustered into four and five genotypes respectively. One new subgroup A and three new subgroup B genotypes were identified. Different genotypes co-circulated in every season. Different circulation patterns were identified for group A and B isolates. Subgroup A revealed more variability and displacement of genotypes while subgroup B remained more consistent.
在南非一家三级医院对呼吸道合胞病毒（RSV）的分子流行病学进行了连续四个季节（1997 - 2000年）的研究：通过逆转录聚合酶链反应（RT-PCR）对225株分离株进行亚组分型，并对所得产物进行测序。A亚组在两个季节中占主导地位，而在其他季节中A亚组和B亚组大致等量共同流行。将G蛋白C末端的核苷酸序列与先前定义的RSV基因型的代表性序列进行比较。南非的A亚组和B亚组分离株分别聚为四个和五个基因型。鉴定出一个新的A亚组基因型和三个新的B亚组基因型。不同基因型在每个季节中共同流行。A组和B组分离株呈现出不同的流行模式。A亚组显示出更多的基因型变异性和替代，而B亚组则保持更为一致。
