Department of Pediatrics, Seoul National University College of Medicine, Seoul, 03080, South Korea.
Department of Pediatrics, Seoul National University Children's Hospital, Seoul, 03080, South Korea.
Arch Virol. 2020 May;165(5):1069-1077. doi: 10.1007/s00705-020-04580-z. Epub 2020 Mar 6.
We investigated the molecular epidemiology of respiratory syncytial virus (RSV) isolated from children during 28 consecutive seasons (1990-2018) and the genetic variability of the duplication region of RSV genotypes ON1 and BA in South Korea. RSV was identified using culture-based methods in Hep-2 cells and was grouped as RSV-A or RSV-B by an immunofluorescence assay. The second hypervariable region of the G gene was sequenced for genotyping. The nucleotide and deduced amino acid sequences of the duplication region of RSV ON1 and BA were analyzed. A total of 670 RSV-A and 233 RSV-B isolates were obtained. For RSV-A, the NA1 genotype predominated during the 2004/2005-2011/2012 seasons. The ON1 genotype was first detected in 2011 and has since replaced all other genotypes. For RSV-B, the GB3 genotype predominated during the 1999/2000-2005/2006 seasons, but the BA genotype also replaced all other genotypes of RSV-B after the first season in which it was isolated (2005/2006). In ON1 and BA genotype RSV strains, novel sequence types of the duplication region of the G gene were identified in 50-95% and 33-80% of the isolates, respectively, in each season. The ON1 and BA9 genotypes are responsible for the current epidemics of RSV infection in South Korea. The sequences in the duplication region of the G gene have evolved continuously and might be sufficient for the identification of specific strains of the RSV-A ON1 and RSV-B BA genotypes.
我们调查了呼吸道合胞病毒（RSV）在 28 个连续季节（1990-2018 年）从儿童中分离株的分子流行病学，以及韩国 RSV 基因型 ON1 和 BA 的重复区的遗传变异性。RSV 是通过 Hep-2 细胞的基于培养的方法鉴定的，并通过免疫荧光分析分为 RSV-A 或 RSV-B。对 G 基因的第二高变区进行测序以进行基因分型。分析 RSV ON1 和 BA 的重复区的核苷酸和推导的氨基酸序列。总共获得了 670 株 RSV-A 和 233 株 RSV-B 分离株。对于 RSV-A，NA1 基因型在 2004/2005-2011/2012 季节占主导地位。ON1 基因型于 2011 年首次检测到，此后已取代所有其他基因型。对于 RSV-B，GB3 基因型在 1999/2000-2005/2006 季节占主导地位，但 BA 基因型在首次分离的季节（2005/2006）后也取代了 RSV-B 的所有其他基因型。在 ON1 和 BA 基因型 RSV 株中，G 基因重复区的新序列类型分别在每个季节的 50-95%和 33-80%的分离株中鉴定。ON1 和 BA9 基因型是导致韩国当前 RSV 感染流行的原因。G 基因重复区的序列不断进化，可能足以识别 RSV-A ON1 和 RSV-B BA 基因型的特定菌株。
