西洋接骨木酊剂作用的遗传毒性评估。

Genotoxic evaluation of the effect of Thuya occidentalys tinctures.

作者信息

Valsa J O, Felzenszwalb I

机构信息

Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

出版信息

Braz J Biol. 2001 May;61(2):329-32. doi: 10.1590/s0034-71082001000200017.

DOI:10.1590/s0034-71082001000200017

PMID:11514902

Abstract

Three tinctures samples from extracts of the popular medicinal plant Thuya occidentalis were tested in vitro through two short term tests for measuring the activity of genotoxic chemicals. Using the Salmonella/mammalian-microsome (Mutatest) assay and the SOS-chromotest (induction of beta-galactosidase in Escherichia coli), none of the extract was effective in inducing mutagenesis or beta-galactosidase synthesis (as an indicator of general and early sign of DNA damage), even with metabolization.

摘要

对常见药用植物北美崖柏提取物的三种酊剂样品进行了体外测试，采用两种短期测试来测定遗传毒性化学物质的活性。使用沙门氏菌/哺乳动物微粒体（Mutatest）试验和SOS显色试验（大肠杆菌中β-半乳糖苷酶的诱导），即使经过代谢，提取物均未有效诱导诱变或β-半乳糖苷酶合成（作为DNA损伤的一般和早期迹象的指标）。