Fukiya S, Kodama M, Kito H, Sone T, Tomita F
Department of Molecular Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Biosci Biotechnol Biochem. 2001 Jul;65(7):1464-73. doi: 10.1271/bbb.65.1464.
Mating experiments between Magnaporthe grisea Japanese rice pathogens and Guy11, a hermaphroditic fertile rice pathogen, were done aimed at identification of avirulence genes. A cross named cross 2107 with thirty-six random progenies was obtained. Segregation analyses of genetic markers found that the cross was less suitable for genetic analysis. Backcrosses with cross 2107 progenies and Guy11 were done and another cross named cross 5307 with sixty-five progenies was obtained. A locus controlling kasugamycin resistance named Ksg1R was identified and used for a model case of genetic mapping. Bulked segregant analysis was done to find adjacent RAPD markers for mapping of the gene. Three adjacent markers to Ksg1R were obtained and a genetic map around the Ksg1R was made, but these markers were not located on a single chromosome. These results suggest that genetic analysis to identify a gene locus is available in cross 5307. Infection assay of parental strains of cross 5307 to Japanese differential rice cultivars suggested the possibility of genetic analysis of cultivar specificity toward four rice cultivars: Aichi-asahi, Kusabue, Tsuyuake, and K59.
对稻瘟病菌日本稻瘟病菌株与两性可育稻瘟病菌株Guy11进行了交配实验，目的是鉴定无毒基因。获得了一个名为杂交2107的杂交组合及其36个随机后代。对遗传标记的分离分析发现，该杂交组合不太适合进行遗传分析。对杂交2107的后代与Guy11进行了回交，获得了另一个名为杂交5307的杂交组合及其65个后代。鉴定出一个控制春雷霉素抗性的基因座Ksg1R，并将其用于遗传作图的一个模型案例。进行了混合分组分析法以寻找用于该基因作图的相邻RAPD标记。获得了三个与Ksg1R相邻的标记，并构建了Ksg1R周围的遗传图谱，但这些标记并不位于同一条染色体上。这些结果表明，在杂交5307中进行鉴定基因座的遗传分析是可行的。对杂交5307的亲本菌株接种日本鉴别水稻品种的感染试验表明，有可能对四个水稻品种：爱知旭、草笛、梅雨明和K59进行品种特异性的遗传分析。
