Dioh W, Tharreau D, Notteghem J L, Orbach M, Lebrun M H
Génétique Moléculaire des Champignons Phytopathogènes, Institut de Génétique et Microbiologie, CNRS-URA 2255, Université Paris-Sud, Orsay, France.
Mol Plant Microbe Interact. 2000 Feb;13(2):217-27. doi: 10.1094/MPMI.2000.13.2.217.
Three genetically independent avirulence genes, AVR1-Irat7, AVRI-MedNoi; and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoi and AVR1-Ku86 were closely linked to telomere RFLPs such as marker TelG (6 cM from AVR1-MedNoi) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning.
在涉及稻瘟病菌Magnaporthe grisea的Guy11和2/0/3分离株的杂交中，鉴定出了三个遗传上独立的无毒基因，即AVR1-Irat7、AVRI-MedNoi和AVR1-Ku86。利用76个随机后代，我们构建了一个部分遗传图谱，该图谱使用了重复序列MGL/MGR583和Pot3/MGR586等探针、来自稻瘟病菌遗传图谱的黏粒以及端粒序列寡核苷酸所揭示的限制性片段长度多态性（RFLP）标记。无毒基因AVR1-MedNoi和AVR1-Ku86与端粒RFLP紧密连锁，如标记TelG（距AVR1-MedNoi 6厘摩）和TelF（距AVR1-Ku86 4.5厘摩）。无毒基因AVR1-Irat7与位于1号染色体上的一个黏粒RFLP连锁，并定位在距无毒基因AVR1-CO39 20厘摩处。利用混合分离群体分析法，我们鉴定出了11个与该杂交中分离的无毒基因紧密连锁（0至10厘摩）的随机扩增多态性DNA（RAPD）标记。这些RAPD标记中的大多数对应于已知或新的转座子与单拷贝序列之间的连接片段。在一个亲本分离株中，此类连接或单拷贝RAPD标记的完整序列常常缺失。与无毒基因紧密连锁的RAPD标记的单拷贝序列将用于图位克隆。
