高压液相色谱法同时测定血浆中苯巴比妥和苯妥英钠的方法的建立与评价
Development and evaluation of method for simultaneous determination of phenobarbital and diphenylhydantoin in plasma by high-pressure liquid chromatography.
作者信息
Atwell S H, Green V A, Haney W G
出版信息
J Pharm Sci. 1975 May;64(5):806-9. doi: 10.1002/jps.2600640516.
PMID:1151650
Abstract
A procedure for the simultaneous analysis of diphenylhydantoin and phenobarbital in plasma by high-pressure liquid chromatography was developed and evaluated. Separation is effected on a porous particle silicic acid column with chloroform dioxane-isopropanol-acetic acid (310:9.7:1.0:0.1 by volume) and is monitored at 254 nm. Results of the procedure were compared with results of a GLC assay.
摘要
建立并评估了一种用高压液相色谱法同时分析血浆中苯妥英和苯巴比妥的方法。在填充有多孔颗粒硅酸的色谱柱上，以氯仿-二氧六环-异丙醇-乙酸（体积比为310:9.7:1.0:0.1）为流动相进行分离，并于254nm处进行监测。将该方法的结果与气相色谱法测定结果进行了比较。
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