电子捕获气相色谱法测定生物体液中一种新型抗心律失常药物α,α-二甲基-4-(α,α,β,β-四氟苯乙基)苄胺
Electron-capture GLC determination of a new antiarrhythmic agent, alpha,alpha-dimethyl-4-(alpha,alpha,beta,beta-tetrafluorophenethyl) benzylamine, in biological fluids.
作者信息
Zacchei A G, Weidner L
出版信息
J Pharm Sci. 1975 May;64(5):814-8. doi: 10.1002/jps.2600640518.
A highly specific and sensitive GLC method was developed for the analysis of alpha,alpha-dimethyl-4-(alpha,alpha,beta,beta-tetrafluorophenethyl) benzylamine, a new orally active antiarrhythmic drug, in biological fluids. The procedure involves the addition of an internal standard, 4-(alpha,alpha,beta,beta-tetrafluorophenethyl)benzylamine, to the plasma or urine samples followed by extraction of the drugs into benzene at pH 8. The extracted amines are converted to the trifluoroacetyl derivatives (characterized by GLG-mass spectrometry), chromatographed, and detected with a 63Ni electron-capture detector. The sensitivity of the method is such that 10 ng of alpha,alpha-dimethyl-4-(alpha,alpha,beta,beta-tetrafluorophenethyl)benzylamine/ml of plasma can be analyzed. These levels are suitable for the analysis of samples obtained following a therapeutic dose.
已开发出一种高特异性和高灵敏度的气相色谱法，用于分析生物流体中的α,α-二甲基-4-(α,α,β,β-四氟苯乙基)苄胺，这是一种新型口服活性抗心律失常药物。该方法包括向血浆或尿液样品中加入内标物4-(α,α,β,β-四氟苯乙基)苄胺，然后在pH 8的条件下将药物萃取到苯中。萃取得到的胺类转化为三氟乙酰衍生物（通过气相色谱-质谱联用进行表征），进行色谱分析，并用63Ni电子捕获检测器进行检测。该方法的灵敏度使得能够分析血浆中10 ng/ml的α,α-二甲基-4-(α,α,β,β-四氟苯乙基)苄胺。这些水平适用于分析治疗剂量后获得的样品。
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