Martinez J R, Quissell D O, Wood D L, Giles M
J Pharmacol Exp Ther. 1975 Aug;194(2):384-95.
Rats treated with 0.5 mg/kg of reserpine per day for 7 days were anesthetized and submaxillary saliva was collected and analyzed for Na+, K+, Ca++ and protein concentrations. Salivary secretion was elicited by i.p. injections of carbamylcholine (50-100 mug/kg), phenylephrine (5 mg/kg) and isoproterenol (10 mg/rat). Saliva was also collected from untreated controls. Submaxillary glands were excised from both groups of animals at the termination of the secretory response, homogenized and analyzed. Glands from other animals were removed in the resting state and similarly processed. Pretreatment with reserpine resulted in decreased volumes of salvia and in elevated salivary concentrations of Ca++ and protein. Saliva from the reserpine-treated animals secreted in response to carbamylcholine had higher concentrations of Na+ and K+ than control saliva, particularly at the low rates of flow. Saliva secreted after stimulation with the two sympathomimetic secretagogues had lower concentrations of these two ions. Resting glands from the treated animals showed significant elevations in protein and Ca++ content and a significant decrease in K+ content. At the end of the secretory response to the three secretagogues, glands from treated animals showed a significantly higher Na+ content and a significantly lower K+ content than control glands. It is concluded that pretreatment with reserpine alters the secretory response of the rat submaxillary gland to both parasympathomimetic and sympathomimetic stimulation. This alteration results from a toxic lesion caused by reserpine in the salivary cells, which involves changes in their permeability to ions and in their energy resources. These in turn, result in an abnormal stimulus-secretion coupling mechanism. The possibility that the toxic lesion is related to alterations in Ca++ homeostasis is discussed.
每天用0.5毫克/千克利血平处理大鼠7天，然后将其麻醉，收集颌下唾液并分析其中Na+、K+、Ca++和蛋白质的浓度。通过腹腔注射氨甲酰胆碱（50 - 100微克/千克）、去氧肾上腺素（5毫克/千克）和异丙肾上腺素（10毫克/只大鼠）来引发唾液分泌。也从未经处理的对照组收集唾液。在分泌反应结束时，从两组动物身上切除颌下腺，匀浆并进行分析。从其他动物身上在静止状态下切除腺体并进行类似处理。利血平预处理导致唾液量减少，唾液中Ca++和蛋白质浓度升高。利血平处理的动物对氨甲酰胆碱反应分泌的唾液中Na+和K+浓度高于对照唾液，尤其是在低流速时。用两种拟交感神经分泌刺激物刺激后分泌的唾液中这两种离子的浓度较低。处理动物的静止腺体显示蛋白质和Ca++含量显著升高，K+含量显著降低。在对三种分泌刺激物的分泌反应结束时，处理动物的腺体显示Na+含量显著高于对照腺体，K+含量显著低于对照腺体。得出的结论是，利血平预处理改变了大鼠颌下腺对拟副交感神经和拟交感神经刺激的分泌反应。这种改变是由利血平在唾液细胞中引起的毒性损伤导致的，这涉及它们对离子的通透性和能量资源的变化。这些反过来又导致异常的刺激 - 分泌偶联机制。讨论了毒性损伤与Ca++稳态改变相关的可能性。
