Huys G, Kämpfer P, Swings J
Laboratory of Microbiology, Ghent University, Belgium.
Syst Appl Microbiol. 2001 Jul;24(2):177-82. doi: 10.1078/0723-2020-00038.
Previously, a DNA fingerprinting study based on Amplified Fragment Length Polymorphism (AFLP) analysis has revealed a possible genotypic resemblance of the species Aeromonas ichthiosmia and Aeromonas allosaccharophila to Aeromonas veronii (Huys et al., Int. J. Syst. Bacteriol. 46, 572-580 [19961). Currently, two genotypically indistinguishable biovars are known to exist in the latter species, namely A. veronii biovar sobria and A. veronii biovar veronii. In the current study, new DNA-DNA hybridization experiments showed that the type strain of A. ichthiosmia, LMG 12645T (= DSM 6393T), and that of A. allosaccharophila, LMG 14059T (= CECT 4199T), were 84-96% and 78-82% related to A. veronii strain LMG 9075T (= ATCC 35624T), respectively. Based upon phenotypic characterization including a total of 151 tests, the type strain of A. ichthiosmia could be clearly allocated to A. veronii biovar sobria. On the other hand, the three strains constituting the species A. allosaccharophila were found to be phenotypically heterogeneous. None of these strains clearly fitted the biochemical description of either of the two A. veronii biovars or tightly clustered with any of the A. veronii reference strains. On the basis of published taxonomic evidence (including AFLP and phylogenetic data) and the newly reported results, there is compiling evidence to conclude that A. ichthiosmia Schubert et al. 1990 is a later synonym of A. veronii Hickman-Brenner et al. 1987. However, due to the lack of agreement encountered between the new DNA reassociation results and previously reported DNA homology and phylogenetic data, a conclusive proposal on the genotypic position of A. allosaccharophila should await further studies.
此前，一项基于扩增片段长度多态性（AFLP）分析的DNA指纹图谱研究表明，鱼害气单胞菌（Aeromonas ichthiosmia）和嗜异麦芽糖气单胞菌（Aeromonas allosaccharophila）在基因型上可能与维罗纳气单胞菌（Aeromonas veronii）相似（Huys等人，《国际系统细菌学杂志》46, 572 - 580 [1996]）。目前已知后一个物种存在两个基因型无法区分的生物变种，即维罗纳气单胞菌温和生物变种（A. veronii biovar sobria）和维罗纳气单胞菌维罗纳生物变种（A. veronii biovar veronii）。在当前研究中，新的DNA - DNA杂交实验表明，鱼害气单胞菌的模式菌株LMG 12645T（= DSM 6393T）以及嗜异麦芽糖气单胞菌的模式菌株LMG 14059T（= CECT 4199T），与维罗纳气单胞菌菌株LMG 9075T（= ATCC 35624T）的相关性分别为84 - 96%和78 - 82%。基于包括总共151项测试的表型特征分析，鱼害气单胞菌的模式菌株可明确归为维罗纳气单胞菌温和生物变种。另一方面，构成嗜异麦芽糖气单胞菌物种的三株菌株在表型上具有异质性。这些菌株均未明确符合两种维罗纳气单胞菌生物变种的生化描述，也未与任何维罗纳气单胞菌参考菌株紧密聚类。根据已发表的分类学证据（包括AFLP和系统发育数据）以及新报告的结果，有越来越多的证据表明1990年的鱼害气单胞菌（A. ichthiosmia Schubert et al.）是1987年的维罗纳气单胞菌（A. veronii Hickman - Brenner et al.）的晚出同义词。然而，由于新的DNA重结合结果与先前报道的DNA同源性和系统发育数据之间存在不一致，关于嗜异麦芽糖气单胞菌基因型位置的确切提议应等待进一步研究。
