嗜异种糖气单胞菌菌株间的DNA相关性及气单胞菌属的DNA杂交群

DNA relatedness among Aeromonas allosaccharophila strains and DNA hybridization groups of the genus Aeromonas.

作者信息

Esteve C, Gutiérrez M C, Ventosa A

机构信息

Departamento de Microbiología y Ecología, Facultad de Ciencias Biológicas, Universitat de València, Burjasot, Spain.

出版信息

Int J Syst Bacteriol. 1995 Apr;45(2):390-1. doi: 10.1099/00207713-45-2-390.

DOI:10.1099/00207713-45-2-390

PMID:7727276

Abstract

The genomic relatedness among three Aeromonas allosaccharophila strains, including the type strain, and other Aeromonas type and reference strains that were assigned to DNA hybridization groups was estimated by DNA-DNA hybridization (competition procedure using a membrane method). All A. allosaccharophila strains were highly related (70 to 100%) to strains 289T (= CECT 4199T) and ATCC 35942. Type strains of other validated Aeromonas species, reference strains of DNA groups 8 and 11, and the Aeromonas sp. strain ATCC 43946 (enteric group 501) were 0 to 41% related to A. allosaccharophila 289T and ATCC 35942. The G+Cs content of A. allosaccharophila strains were in the range 55.9 to 57.3 mol%. The G+C content of the type strain of this species was 56.9 mol%, a value somewhat lower than that reported in the original description.

摘要

通过DNA-DNA杂交（使用膜法的竞争程序），对包括模式菌株在内的三株嗜糖气单胞菌菌株与其他被归入DNA杂交组的气单胞菌模式菌株及参考菌株之间的基因组相关性进行了评估。所有嗜糖气单胞菌菌株与菌株289T（=CECT 4199T）和ATCC 35942高度相关（70%至100%）。其他已验证的气单胞菌物种的模式菌株、DNA组8和11的参考菌株以及气单胞菌属菌株ATCC 43946（肠道菌群501）与嗜糖气单胞菌289T和ATCC 35942的相关性为0%至41%。嗜糖气单胞菌菌株的G+C含量在55.9至57.3 mol%范围内。该物种模式菌株的G+C含量为56.9 mol%，该值略低于原始描述中报道的值。