Todoriki M, Oki S, Matsuyama S-I, Urabe I, Yomo T
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita City, Japan.
J Biol Phys. 2002 Dec;28(4):793-7. doi: 10.1023/A:1021263427826.
Two well-characterized and phylogenetically diverse species, Escherichiacoli and Dictyostelium discoideum, were used as the modelorganisms. When the two species were mixed and allowed to grow onminimal agar plates at 22 (°)C, instead of the predator Dictyostelium exterminating E.coli, the two species remarkablyachieved a state of stable coexistence in about two weeks. In addition, theemerged colonies housing the coexisting species have a mucoidal naturethat is distinctive from its origin. The simplicity of the system and the shorttime span for the two species to develop the coexistence state, that isproven stable and reproducible on laboratory conditions, hence, providesa new model system for the study of symbiosis, particularly with referenceto the initial stages.
两种特征明确且系统发育上具有多样性的物种，大肠杆菌和盘基网柄菌，被用作模式生物。当将这两种物种混合并在22℃的基本琼脂平板上培养时，并没有出现捕食者盘基网柄菌消灭大肠杆菌的情况，相反，这两种物种在大约两周内显著地实现了稳定共存的状态。此外，容纳共存物种的新出现的菌落具有一种与其起源不同的黏液性质。该系统的简单性以及这两种物种形成共存状态的短时间跨度，在实验室条件下已被证明是稳定且可重复的，因此，为共生研究，特别是关于共生初始阶段的研究，提供了一个新的模型系统。
