Uhríková D, Kucerka N, Islamov A, Gordeliy V, Balgavý P
Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia.
Gen Physiol Biophys. 2001 Jun;20(2):183-9.
Mixtures of N-dodecyl-N,N-dimethylamine N-oxide (DDAO) and 1,2-dioleoylphosphatidyl choline (DOPC) in chloroform/methanol were evaporated, dried and hydrated in excess 2H2O. Aqueous dispersions thus prepared were extruded through polycarbonate filter with pores of diameter 500A. These samples were studied using small-angle neutron scattering. DDAO destabilizes the bilayer in unilamellar liposomes and solubilizes it into mixed micelles whose shape changes with the DDAO : DOPC molar ratio. Bilayers or/and bilayer fragments have been observed up to DDAO : DOPC = 1.5, rod-like particles (tubular, cylindric micelles) at 2.5 < DDAO : DOPC < 3.5, and transition to globular particles (spheroid micelles) at DDAO: DOPC > 4. In bilayers or/and bilayer fragments, DDAO modulates the thickness of the bilayer.
将氯仿/甲醇中的N-十二烷基-N,N-二甲基氧化胺（DDAO）和1,2-二油酰基磷脂酰胆碱（DOPC）混合物蒸发、干燥并在过量的重水（2H₂O）中进行水合。如此制备的水分散体通过孔径为500埃的聚碳酸酯滤膜挤出。使用小角中子散射对这些样品进行研究。DDAO会破坏单层脂质体中的双层结构，并将其溶解成混合胶束，其形状会随DDAO与DOPC的摩尔比而变化。在DDAO与DOPC的摩尔比达到1.5之前观察到双层或/和双层片段，在2.5 < DDAO与DOPC的摩尔比 < 3.5时观察到棒状颗粒（管状、圆柱形胶束），在DDAO与DOPC的摩尔比 > 4时转变为球状颗粒（球状胶束）。在双层或/和双层片段中，DDAO会调节双层的厚度。
