Park J Y, Gemmell C H, Davies J E
Bone Interface Group, Faculty of Dentistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ont, Canada.
Biomaterials. 2001 Oct;22(19):2671-82. doi: 10.1016/s0142-9612(01)00009-6.
Endosseous implants initially come into contact with blood. Thus, the nature of the interactions between blood and implanted endosseous implants may influence subsequent bone healing events in the peri-implant healing compartment. We conducted studies to address the following question: Does implant surface microtexture modulate platelet activity? We used commercially pure Ti (cpTi) disks with four different surface finishes: dual acid-etched (DAE), 320 grit (320G) abraded, machined, and p1200 polished cpTi. Surfaces were characterized by scanning electron microscopy (SEM) and optical profilometry. The DAE and 320G surfaces presented more complex microtextures than the machined or polished surfaces. Platelet activities were measured by quantifying platelet adherence, platelet-derived microparticle (MP) formation, and P-selectin expression as function of surface type. Platelet adhesion, measured using a lactate dehydrogenase (LDH) assay. was increased on DAE and 320G surfaces compared to machined and polished surfaces (p < 0.05). M P formation and P-selectin expression, assayed by flow cytometry, also showed increased activation of platelets on DAE and 320G surfaces. Because increased activation of platelets may lead to up-regulation of osteogenic responses during bone healing, these results may explain the enhanced osteoconductivity known to occur with DAE cpTi surfaces in comparison with machined cpTi surfaces.
骨内植入物最初会与血液接触。因此，血液与植入的骨内植入物之间相互作用的性质可能会影响种植体周围愈合区域随后的骨愈合过程。我们开展了研究以解决以下问题：种植体表面微观纹理是否会调节血小板活性？我们使用了具有四种不同表面处理的商业纯钛（cpTi）圆盘：双酸蚀刻（DAE）、320目（320G）研磨、机械加工以及p1200抛光的cpTi。通过扫描电子显微镜（SEM）和光学轮廓仪对表面进行表征。与机械加工或抛光表面相比，DAE和320G表面呈现出更复杂的微观纹理。通过量化血小板黏附、血小板衍生微粒（MP）形成以及P-选择素表达作为表面类型的函数来测量血小板活性。使用乳酸脱氢酶（LDH）测定法测量的血小板黏附在DAE和320G表面上比机械加工和抛光表面增加（p < 0.05）。通过流式细胞术测定的MP形成和P-选择素表达也显示DAE和320G表面上血小板的活化增加。由于血小板活化增加可能导致骨愈合过程中成骨反应的上调，这些结果可能解释了与机械加工的cpTi表面相比，DAE cpTi表面已知具有增强的骨传导性。
