Paquette D M, Banks P R
Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada.
Electrophoresis. 2001 Aug;22(12):2391-7. doi: 10.1002/1522-2683(200107)22:12<2391::AID-ELPS2391>3.0.CO;2-B.
Solution-phase immunoassays based on capillary electrophoresis (CE) separations have been shown to be rapid and simple to perform. The potential for sample matrix interference and incompatibility with multiplexing conditions for antibody detection when dealing with real samples, however, has prompted the development of an assay that utilizes an immunosubtraction methodology. A model assay for the detection of specific antibodies that relies on solid-phase extraction, CE and laser-induced fluorescence (LIF) detection is described. The method, called immunocapture-immunosubtraction (ICIS), incorporates an antibody capture/purification protocol using magnetic particles. The detection of specific antibodies is achieved by CE-LIF analysis of a probe solution following incubation with the captured antibodies. As an example of the ICIS assay's capabilities, the relative quantification of anti-fluorescein in serum is presented.
基于毛细管电泳（CE）分离的溶液相免疫测定已被证明操作快速且简单。然而，在处理实际样品时，样品基质干扰的可能性以及与抗体检测的多重条件不兼容性促使了一种利用免疫减法方法的测定方法的发展。本文描述了一种基于固相萃取、CE和激光诱导荧光（LIF）检测的特异性抗体检测模型测定法。该方法称为免疫捕获-免疫减法（ICIS），采用了使用磁性颗粒的抗体捕获/纯化方案。通过在与捕获的抗体孵育后对探针溶液进行CE-LIF分析来实现特异性抗体的检测。作为ICIS测定法能力的一个例子，给出了血清中抗荧光素的相对定量。
