Ferguson P L, Grange A H, Brumley W C, Donnelly J R, Farley J W
US Environmental Protection Agency, National Exposure Research Laboratory, Environmental Sciences Division, Las Vegas, NV 89193-3478, USA.
Electrophoresis. 1998 Sep;19(12):2252-6. doi: 10.1002/elps.1150191234.
Capillary electrophoresis (CE) has been applied to the determination of the groundwater migration tracer dye fluorescein based on laser-induced fluorescence (LIF) detection and compared to determinations obtained with traditional spectrofluorimetry. Detection limits of injected dye in the low parts per trillion (ppt) ranges have been accomplished with both CE/LIF based on the Ar ion laser and with a spectrofluorimeter. This approach was used for a real-world problem in determining groundwater migration between adjacent Resource Conservation and Recovery Act (RCRA) and Superfund sites by the Environmental Sciences Division in response to regional needs and as application of new analytical tools under development. Fluorescent dye was injected into source wells and then was determined in monitoring wells by extracting pads that adsorbed the dye or by directly determining the dye in the water using solid-phase extraction (SPE), a preconcentration technique. The approaches based on CE/LIF exhibits increased specificity over existing approaches due to the separation and unique migration time of the dye. Additional studies were aimed at achieving sub-ppt levels in the water using solid-phase extraction and field-amplified injection techniques.
毛细管电泳（CE）已基于激光诱导荧光（LIF）检测应用于地下水中迁移示踪染料荧光素的测定，并与传统荧光光谱法的测定结果进行了比较。基于氩离子激光的CE/LIF和荧光光谱仪都实现了在低万亿分之一（ppt）范围内注入染料的检测限。该方法被用于解决一个实际问题，即环境科学部门应区域需求，在确定相邻的《资源保护与回收法》（RCRA）和超级基金场地之间的地下水迁移时，作为正在开发的新分析工具的应用。将荧光染料注入源井，然后通过吸附染料的萃取垫在监测井中进行测定，或者使用预浓缩技术固相萃取（SPE）直接测定水中的染料。基于CE/LIF的方法由于染料的分离和独特迁移时间，比现有方法具有更高的特异性。额外的研究旨在使用固相萃取和场放大进样技术实现水中亚ppt水平的检测。
