Edhemović I, Snoj M, Kljun A, Golouh R
Department of Surgical Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000 Ljubljana, Slovenia.
Eur J Surg Oncol. 2001 Sep;27(6):545-8. doi: 10.1053/ejso.2001.1134.
Group II phospholipase A(2)(PLA(2)) is an enzyme important in malignant transformation and in the invasion process of malignant cells. The aim of the present study was to investigate the expression of group II PLA(2)in the cancers of the colorectum, peritumoural mucosa and in the mucosa distant from the tumour.
Resection specimens from 57 patients with colorectal carcinoma (caecum 10, ascending 10, transverse 10, sigmoid colon nine, and rectum 18) were analysed immunohistochemically. Histological slides from paraffin blocks were stained by the human monoclonal group II PLA(2)antibody ('Upstate Biotechnology', Lake Placid, NY 12946, USA. Antibody Class: IgG1k. Immunogen: HPC purifed human sperm phospholipase A(2)- 14 kDa enzyme) using the standard DAKO peroxidase-labelled streptavidin-biotin method by TechMate 500 stainer. Group II PLA(2)expression was evaluated semi-quantitatively according to the extensivity and intensivity of the positive cells. For statistical evaluation the Kruskal-Wallis one way analysis of variance on ranks and the Mann-Whitney rank sum tests were used.
The highest expression of group II PLA(2)was found in the peritumoural mucosa (median 4.00), much lower in the mucosa distal from the tumour (median 0.70) and almost no activity in the tumour itself (median 0.00), all differences were statistically significant (all pairwise multiple comparison procedures - Dunn's Method P<0.05). The expression of group II PLA(2)was higher in the left colon and rectum than in the right colon (Mann-Whitney rank sum test P<0.05).
Our results suggest that there is variation of the group II PLA(2)expression throughout the mucosa and tumours of the colorectum, which might reflect the progression of neoplastic process.
II 型磷脂酶 A₂（PLA₂）是一种在恶性转化及恶性细胞侵袭过程中起重要作用的酶。本研究旨在调查 II 型 PLA₂ 在结直肠癌、肿瘤周围黏膜及远离肿瘤的黏膜中的表达情况。
对 57 例结直肠癌患者（盲肠 10 例、升结肠 10 例、横结肠 10 例、乙状结肠 9 例、直肠 18 例）的切除标本进行免疫组织化学分析。石蜡块制成的组织切片，采用标准的 DAKO 过氧化物酶标记链霉亲和素 - 生物素法，由 TechMate 500 染色仪，使用人源单克隆 II 型 PLA₂ 抗体（“Upstate Biotechnology”，美国纽约州普拉西德湖 12946。抗体类别：IgG1k。免疫原：HPC 纯化的人精子磷脂酶 A₂ - 14 kDa 酶）进行染色。根据阳性细胞的范围和强度对 II 型 PLA₂ 的表达进行半定量评估。统计分析采用 Kruskal - Wallis 秩和检验及 Mann - Whitney 秩和检验。
II 型 PLA₂ 在肿瘤周围黏膜中的表达最高（中位数为 4.00），在远离肿瘤的黏膜中低得多（中位数为 0.70），而在肿瘤本身几乎无活性（中位数为 0.00），所有差异均具有统计学意义（所有两两多重比较程序 - Dunn 法 P<0.05）。II 型 PLA₂ 在左半结肠和直肠中的表达高于右半结肠（Mann - Whitney 秩和检验 P<0.05）。
我们的结果表明，II 型 PLA₂ 在整个结直肠黏膜和肿瘤中的表达存在差异，这可能反映了肿瘤形成过程的进展。
