丝裂霉素C治疗眼表鳞状上皮肿瘤后的印迹细胞学检查
Impression cytology following mitomycin C therapy for ocular surface squamous neoplasia.
作者信息
McKelvie P A, Daniell M
机构信息
Department of Anatomical Pathology, St Vincent's Hospital, Melbourne, Victoria, Australia.
出版信息
Br J Ophthalmol. 2001 Sep;85(9):1115-9. doi: 10.1136/bjo.85.9.1115.
BACKGROUND/AIMS: Topical mitomycin C (MMC) therapy has been used for treatment of ocular surface squamous neoplasia (OSSN) since 1994. Relatively few studies have reported the cellular changes in ocular surface following MMC.
METHODS
Impression cytology was studied in four patients with ocular surface squamous neoplasia, either primary or recurrence after previous excisional biopsy. The authors studied samples obtained using Millipore filters at intervals between 4 and 17 weeks after commencement of MMC, and compared them with pretreatment cytology.
RESULTS
MMC induced changes of cytomegaly, cytoplasmic vacuolation, nucleomegaly with nuclear wrinkling, and binucleation or multinucleation were seen in some cells in all samples. However, nuclear/cytoplasmic (N/C) ratio in these enlarged cells was normal. These changes mimicked those seen following radiation therapy in uterine cervix. Changes of increased nuclear and cell size with increased N/C ratio were seen in some dysplastic cells. The predominant form of cell death was apoptosis with fewer cells showing necrosis.
CONCLUSIONS
MMC appears to produce cell death in OSSN by apoptosis and necrosis. Cellular changes related to MMC mimic those caused by radiation-cytomegaly, nucleomegaly, and vacuolation. MMC related changes may persist in ocular surface epithelium for at least 8 months following MMC therapy.
背景/目的：自1994年以来，局部应用丝裂霉素C（MMC）治疗眼表鳞状上皮肿瘤（OSSN）。相对较少的研究报道了MMC治疗后眼表的细胞变化。
方法
对4例眼表鳞状上皮肿瘤患者进行印片细胞学研究，这些患者均为原发性或先前切除活检后复发。作者研究了在开始MMC治疗后4至17周期间使用密理博滤膜获得的样本，并将其与治疗前的细胞学进行比较。
结果
在所有样本的一些细胞中均可见MMC诱导的细胞肿大、细胞质空泡化、核肿大伴核皱缩以及双核或多核现象。然而，这些增大细胞的核/质（N/C）比值正常。这些变化类似于子宫颈放疗后的变化。在一些发育异常的细胞中可见核和细胞大小增加以及N/C比值增加的变化。细胞死亡的主要形式是凋亡，显示坏死的细胞较少。
结论
MMC似乎通过凋亡和坏死导致OSSN中的细胞死亡。与MMC相关的细胞变化类似于辐射引起的变化——细胞肿大、核肿大和空泡化。MMC治疗后，相关变化可能在眼表上皮中持续至少8个月。
Zotero 插件