Cole A M, Kim Y H, Tahk S, Hong T, Weis P, Waring A J, Ganz T
Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90095, USA.
FEBS Lett. 2001 Aug 24;504(1-2):5-10. doi: 10.1016/s0014-5793(01)02731-4.
The human airways are protected from pathogenic colonization by a blanket of fluid impregnated with innate antimicrobial effector molecules. Among several previously uncharacterized components, we isolated a peptide that had activity primarily targeting Gram-negative bacteria. We named the peptide 'calcitermin' since its amino acid sequence and mass were equivalent to the 15 C-terminal residues of the S100 protein, calgranulin C. The antimicrobial activity of calcitermin was enhanced in acidic buffers (pH 5.4) and in the presence of micromolar concentrations of ZnCl(2). Analysis revealed a putative zinc-binding consensus sequence as well as an alpha-helical conformation in structure-promoting solvents.
人体气道受到一层充满先天性抗菌效应分子的液体的保护，免受病原体定植。在几个先前未被表征的成分中，我们分离出一种主要靶向革兰氏阴性菌的具有活性的肽。我们将该肽命名为“钙调素”，因为其氨基酸序列和质量与S100蛋白钙粒蛋白C的15个C端残基相当。在酸性缓冲液（pH 5.4）和微摩尔浓度的ZnCl₂存在下，钙调素的抗菌活性增强。分析揭示了一个假定的锌结合共有序列以及在结构促进溶剂中的α-螺旋构象。
