Zhang X H, Sun H M, Yuan J Q
International Intraocular Implant Training Centre, Tianjin Medical University, Tianjin, People's Republic of China.
J Cataract Refract Surg. 2001 Aug;27(8):1303-9. doi: 10.1016/s0886-3350(00)00833-6.
To examine extracellular matrix (ECM) production of lens epithelial cells (LECs) and their regulation by cytokines.
Research Laboratory, International Intraocular Implant Training Center, Tianjin Medical University, People's Republic of China.
Bovine LECs were cultured with or without tumor necrosis factor alpha (TNF-alpha) and transforming growth factor beta2 (TGF-beta2). Immunohistochemistry and in situ hybridization were used to detect the expression of ECM. The slides were quantified by computer analysis.
Laminin expression was increased by both TNF-alpha and TGF-beta2. The expression of type IV collagen at the mRNA level was upregulated by TNF-alpha and TGF-beta2. Its protein expression was promoted by only TNF-alpha.
Type IV collagen and laminin are the main components of the lens capsule. Their production of LECs could be increased by cytokines derived from the aqueous humor or LECs. This may lead to ECM deposition and capsule fibrosis during the development of posterior capsule opacification.
研究晶状体上皮细胞（LECs）的细胞外基质（ECM）产生及其受细胞因子的调控。
中国天津医科大学国际眼内植入培训中心研究实验室。
将牛LECs在有或无肿瘤坏死因子α（TNF-α）和转化生长因子β2（TGF-β2）的条件下培养。采用免疫组织化学和原位杂交检测ECM的表达。通过计算机分析对玻片进行定量。
TNF-α和TGF-β2均使层粘连蛋白表达增加。TNF-α和TGF-β2使IV型胶原mRNA水平的表达上调。仅TNF-α促进其蛋白表达。
IV型胶原和层粘连蛋白是晶状体囊膜的主要成分。房水或LECs来源的细胞因子可增加LECs对它们的产生。这可能在后囊膜混浊形成过程中导致ECM沉积和囊膜纤维化。
