在四氧嘧啶糖尿病模型过程中的胰岛素、葡萄糖、胆固醇水平及溶酶体酶活性。
The insulin, glucose and cholesterol level and activity of lysosomal enzymes in the course of the model alloxan diabetes.
作者信息
Witek B, Król T, Kołataj A, Ochwanowska E, Stanisławska I, Slewa A
机构信息
Department of Genetics, Institute of Biology, Swietokrzyska Academy, Poland.
出版信息
Neuro Endocrinol Lett. 2001 Aug;22(4):238-42.
OBJECTIVES
The study was carried out on fifty male rabbits of the New Zealand White breed. Diabetes was caused by a single, intravenous alloxan injection. Rabbits which had glycaemia 7th day after the alloxan administration higher than 11 millimol/litre were selected for the studies. They were divided into 5 groups: I - control (without diabetes); II - 3-week diabetes; III - 6-week diabetes; IV - 3-month diabetes; V - 6-month diabetes.
METHODS
In control and experimental rabbits the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, lysosomal acid phosphatase, leucine aminopeptidase, cathepsin D, and lysosomal arylesterase was determined in lysosomal fractions of the liver and kidney.
RESULTS
Alloxan caused lowering of the activity of all the investigated enzymes in the kidney and liver except lysosomal arylesterase.
CONCLUSION
Alloxan injection caused a significant increase in the activity of all the investigated enzymes. The advisable lysosomal enzymes may be useful for the monitoring of the course and effectiveness of diabetes therapy.
目的
对50只新西兰白兔雄性进行研究。通过单次静脉注射四氧嘧啶诱导糖尿病。选择在四氧嘧啶给药后第7天血糖高于11毫摩尔/升的兔子进行研究。将它们分为5组：I组 - 对照组（无糖尿病）；II组 - 3周糖尿病组；III组 - 6周糖尿病组；IV组 - 3个月糖尿病组；V组 - 6个月糖尿病组。
方法
在对照组和实验兔中，测定肝脏和肾脏溶酶体部分中β-葡萄糖醛酸酶、N-乙酰-β-氨基葡萄糖苷酶、溶酶体酸性磷酸酶、亮氨酸氨肽酶、组织蛋白酶D和溶酶体芳基酯酶的活性。
结果
除溶酶体芳基酯酶外，四氧嘧啶导致肾脏和肝脏中所有研究酶的活性降低。
结论
注射四氧嘧啶导致所有研究酶的活性显著增加。所检测的溶酶体酶可能有助于监测糖尿病治疗的过程和效果。
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