Turkoglu Oya, Emingil Gulnur, Eren Gülnihal, Atmaca Harika, Kutukculer Necil, Atilla Gül
Department of Periodontology, School of Dentistry, Ege University, Bornova, 35100, Izmir, Turkey.
Department of Biology, School of Science and Letters, Celal Bayar University, Manisa, Turkey.
Clin Oral Investig. 2017 Apr;21(3):763-769. doi: 10.1007/s00784-016-1834-z. Epub 2016 Apr 30.
hCAP18/LL-37 is an endogenous antibiotic having a role in innate immunity. The aim of the present study was to evaluate serum and gingival crevicular fluid (GCF) hCAP18/LL-37 levels in patients with generalized aggressive periodontitis (G-AgP).
Twenty-six G-AgP patients, 24 gingivitis patients, and 25 healthy subjects were included in this study. Periodontal parameters including probing depth, clinical attachment level, plaque index, and papilla bleeding index were recorded. GCF and serum hCAP18/LL-37 levels were analyzed by enzyme-linked immunosorbent assay.
GCF hCAP18/LL-37 level was significantly higher in G-AgP compared to others (p = 0.038, p < 0.001). Gingivitis patients had significantly higher GCF hCAP18/LL-37 levels than controls (p < 0.001). No significant differences were observed in serum hCAP18/LL-37 levels among the study groups (p = 0.524). While there were positive correlations between GCF hCAP18/LL-37 levels and periodontal parameters of sampling sites (p < 0.005), no significant correlation was observed between serum hCAP18/LL-37 levels and whole-mouth periodontal parameters (p > 0.05).
Increased levels of GCF hCAP18/LL-37 in G-AgP might show that it is abundantly expressed in the presence of periodontal tissue destruction. Serum hCAP18/LL-37 levels do not seem to be related with the presence of G-AgP.
hCAP18/LL-37 antimicrobial peptide might be associated with periodontal tissue destruction in the presence of aggressive periodontitis.
人阳离子抗菌蛋白18/LL-37（hCAP18/LL-37）是一种在天然免疫中发挥作用的内源性抗生素。本研究旨在评估广泛侵袭性牙周炎（G-AgP）患者血清和龈沟液（GCF）中hCAP18/LL-37的水平。
本研究纳入了26例G-AgP患者、24例牙龈炎患者和25名健康受试者。记录牙周参数，包括探诊深度、临床附着水平、菌斑指数和龈乳头出血指数。采用酶联免疫吸附测定法分析GCF和血清中hCAP18/LL-37的水平。
与其他组相比，G-AgP患者的GCF中hCAP18/LL-37水平显著更高（p = 0.038，p < 0.001）。牙龈炎患者的GCF中hCAP18/LL-37水平显著高于对照组（p < 0.001）。研究组之间血清hCAP18/LL-37水平未观察到显著差异（p = 0.524）。虽然GCF中hCAP18/LL-37水平与取样部位的牙周参数之间存在正相关（p < 0.005），但血清hCAP18/LL-37水平与全口牙周参数之间未观察到显著相关性（p > 0.05）。
G-AgP患者GCF中hCAP18/LL-37水平升高可能表明其在牙周组织破坏时大量表达。血清hCAP18/LL-37水平似乎与G-AgP的存在无关。
在侵袭性牙周炎存在时，hCAP18/LL-37抗菌肽可能与牙周组织破坏有关。
