Li Q, Lawrence C B, Xing H Y, Babbitt R A, Bass W T, Maiti I B, Everett N P
THRI, University of Kentucky, Lexington 40546-0236, USA.
Planta. 2001 Mar;212(4):635-9. doi: 10.1007/s004250000480.
Magainins are a group of short peptides originally isolated from frog skin and thought to function as a natural defense mechanism against infection due to their antimicrobial properties. The engineered magainin analog peptide Myp30 was found to inhibit spore germination of the oomycete, Peronospora tabacina (Adam) in vitro, and the growth of a bacterial pathogen Erwinia carotovora subsp. carotovora (Jones). Transgenic tobacco (Nicotiana tabacum L.) plants expressing Myp30 were evaluated for resistance to these pathogens. The expression of the peptide only to an extracellular location resulted in significant reduction in sporulation and lesion size due to P. tabacina infection. A significant increase in resistance to the bacterial pathogen was also observed regardless of the targeting location of the peptide.
马盖宁是一组最初从蛙皮中分离出来的短肽，由于其抗菌特性，被认为是一种抵御感染的天然防御机制。经工程改造的马盖宁类似物肽Myp30在体外被发现可抑制卵菌烟草霜霉病菌（Adam）的孢子萌发以及细菌性病原菌胡萝卜软腐欧文氏菌胡萝卜软腐亚种（Jones）的生长。对表达Myp30的转基因烟草（烟草）植株进行了针对这些病原菌的抗性评估。该肽仅在细胞外位置表达，导致因烟草霜霉病菌感染而产生的孢子形成和病斑大小显著降低。无论该肽的靶向位置如何，对细菌性病原菌的抗性也显著增加。
