Xing Hongyan, Lawrence Christopher B, Chambers Orlando, Davies H Maelor, Everett Nicholas P, Li Qingshun Quinn
Kentucky Tobacco Research and Development Center, University of Kentucky, Lexington, 40546, USA.
Planta. 2006 Apr;223(5):1024-32. doi: 10.1007/s00425-005-0143-6. Epub 2005 Nov 24.
Reverse peptide of indolicidin (Rev4), a 13-residue peptide based on the sequence of indolicidin, has been shown to possess both strong antimicrobial and protease inhibitory activities in vitro. To evaluate its efficacy in vivo, we produced and evaluated transgenic tobacco (Nicotiana tabacum L.) and Arabidopsis thaliana [(L.) Heynh.] plants expressing Rev4 with different signal peptide sequences for pathogen resistance. All transgenic plants showed normal growth and development, an indication of no or low cytotoxicity of the peptide. Furthermore, the transgenic plants exhibited elevated resistance to three bacterial and two oomycete pathogens. Interestingly, tobacco plants expressing Rev4 displayed enhanced yield compared to the control as indicated by an increased biomass production by as much as 34% in two field trials. When Rev4 was coexpressed with another antimicrobial peptide, Myp30, the disease resistance levels in the transgenic Arabidopsis were enhanced. These findings suggest the potential of using these peptides to protect plants from microbial pathogens and to enhance yield.
基于吲哚杀菌素序列的13个氨基酸残基的反向肽（Rev4）已被证明在体外具有强大的抗菌和蛋白酶抑制活性。为了评估其体内功效，我们培育并评估了表达带有不同信号肽序列的Rev4的转基因烟草（烟草）和拟南芥，以检测其对病原体的抗性。所有转基因植物均生长发育正常，这表明该肽无细胞毒性或细胞毒性较低。此外，转基因植物对三种细菌病原体和两种卵菌病原体表现出更高的抗性。有趣的是，在两次田间试验中，表达Rev4的烟草植株生物量增加了34%，表明与对照相比产量提高。当Rev4与另一种抗菌肽Myp30共表达时，转基因拟南芥的抗病水平增强。这些发现表明，利用这些肽保护植物免受微生物病原体侵害并提高产量具有潜力。
