临床样本中人乳头瘤病毒检测、分型及定量的新方法。
Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples.
作者信息
Hart K W, Williams O M, Thelwell N, Fiander A N, Brown T, Borysiewicz L K, Gelder C M
机构信息
Section of Infection and Immunity, Department of Medicine, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XX, United Kingdom.
出版信息
J Clin Microbiol. 2001 Sep;39(9):3204-12. doi: 10.1128/JCM.39.9.3204-3212.2001.
Abstract
We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease.
摘要
我们报告了一种基于实时PCR和名为Scorpions的自探测荧光引物的新型人乳头瘤病毒（HPV）检测和分型方法的开发。该技术快速、简单、特异、灵敏，并且能够估计每个细胞的病毒载量。我们报告了对细胞系、活检组织和宫颈细胞刷样本进行的100多次分型反应的结果，与当前的HPV检测和分型参考技术相比，kappa值为0.89。我们还进一步报告了初步数据，表明每个细胞的病毒载量与宫颈疾病分级之间存在关联。
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