HPV 感染、危险因素和病毒载量在墨西哥男大学生中的研究。
HPV infection, risk factors and viral load among Mexican male college students.
机构信息
Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
Colegio de la Frontera Norte, Piedras Negras, Coahuila, Mexico.
出版信息
Braz J Infect Dis. 2014 Jan-Feb;18(1):71-6. doi: 10.1016/j.bjid.2013.05.006. Epub 2013 Sep 19.
OBJECTIVES
To determine the prevalence of HPV and the risky sexual behaviors associated to it in a sample of male college students, taking into account genotype and viral load.
METHODS
From 2002 to 2003, male students from the Autonomous University of Morelos State completed a questionnaire and provided self-collected genital samples to detect and quantify HPV. We performed a bivariate and a multivariate logistic regression analysis to identify correlates associated with the infection and to assess the viral load as a function of the viral infecting type. The fragments of β-globin gene and L1 of HPV, were amplified, purified and cloned, to evaluate viral load.
RESULTS
Among 253 subjects, HPV prevalence was 19.4%, and HPV16 was the most common subtype. History of STIs (OR=4.8; 95% CI 1.2-18.9), contraceptive pill use by female partner (OR=2.6; 95% CI 1.1-6.3) and exchanging sex for money (OR=4.9; 95% CI 1.2-20) were associated to the HPV infection. HPV16 viral load was 7.8 copies (HPV/beta-globin) compared to 0.9 copies for other HPV types.
DISCUSSION
HPV16 displayed the highest viral load, and it was the most prevalent. It was found that using contraceptive pills by female partners was associated with HPV infection.
目的
在男性大学生样本中，确定 HPV 的流行率以及与之相关的危险性行为，同时考虑到基因型和病毒载量。
方法
2002 年至 2003 年，来自莫雷洛斯自治大学的男性学生完成了一份问卷，并提供了自行采集的生殖器样本，以检测和量化 HPV。我们进行了双变量和多变量逻辑回归分析，以确定与感染相关的关联因素，并评估病毒载量作为感染病毒类型的函数。β-球蛋白基因和 HPV 的 L1 片段被扩增、纯化和克隆，以评估病毒载量。
结果
在 253 名受试者中，HPV 患病率为 19.4%，HPV16 是最常见的亚型。性传播感染史（OR=4.8；95%CI 1.2-18.9）、女性伴侣使用避孕药（OR=2.6；95%CI 1.1-6.3）和性交易（OR=4.9；95%CI 1.2-20）与 HPV 感染相关。HPV16 的病毒载量为 7.8 拷贝（HPV/β-球蛋白），而其他 HPV 类型的病毒载量为 0.9 拷贝。
讨论
HPV16 显示出最高的病毒载量，也是最常见的。研究发现，女性伴侣使用避孕药与 HPV 感染相关。
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