吲哚菁绿对培养的人视网膜色素上皮细胞的影响：对黄斑裂孔手术的意义。
Indocyanine green effect on cultured human retinal pigment epithelial cells: implication for macular hole surgery.
作者信息
Sippy B D, Engelbrecht N E, Hubbard G B, Moriarty S E, Jiang S, Aaberg T M, Aaberg T M, Grossniklaus H E, Sternberg P
机构信息
Department of Ophthalmology, Emory University School of Medicine, Atlanta, GA 30322, USA.
出版信息
Am J Ophthalmol. 2001 Sep;132(3):433-5. doi: 10.1016/s0002-9394(01)01061-3.
PURPOSE
To evaluate potential toxic effects of indocyanine green dye on cultured human retinal pigment epithelial cells.
METHODS
Controlled laboratory experiment. Cultured human retinal pigment epithelial cells were exposed to balanced saline solution, balanced saline solution with endoillumination, indocyanine green or indocyanine green with endoillumination. Cells were evaluated by light microscopy, electron microscopy, and a mitochondrial dehydrogenase assay.
RESULTS
Retinal pigment epithelial cells exposed to indocyanine green showed no histologic or ultrastructural changes. Those exposed to indocyanine green alone or indocyanine green plus light demonstrated a significant decrease in mitochondrial enzyme activity (P = 0.0002 and 0.005, respectively).
CONCLUSION
Brief exposure of cultured human retinal pigment epithelial cells to indocyanine green results in decreased mitochondrial enzyme activity but does not appear to influence cellular morphology or ultrastructure.
目的
评估吲哚菁绿染料对培养的人视网膜色素上皮细胞的潜在毒性作用。
方法
对照实验室实验。将培养的人视网膜色素上皮细胞暴露于平衡盐溶液、带内照明的平衡盐溶液、吲哚菁绿或带内照明的吲哚菁绿中。通过光学显微镜、电子显微镜和线粒体脱氢酶测定法对细胞进行评估。
结果
暴露于吲哚菁绿的视网膜色素上皮细胞未显示出组织学或超微结构变化。单独暴露于吲哚菁绿或吲哚菁绿加光照的细胞线粒体酶活性显著降低（分别为P = 0.0002和0.005）。
结论
培养的人视网膜色素上皮细胞短暂暴露于吲哚菁绿会导致线粒体酶活性降低，但似乎不会影响细胞形态或超微结构。
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