Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
Manchester Royal Eye Hospital, Manchester University Hospitals NHS Foundation Trust, Manchester, UK.
Transl Vis Sci Technol. 2023 Jan 3;12(1):5. doi: 10.1167/tvst.12.1.5.
To assess the safety profile of a new lutein-based vitreous dye (LB-VD) formulation compared with various triamcinolone acetonide (TA) formulations with and without subsequent exposure to perfluorodecalin (PFD) in vitro.
Human adult retinal pigment epithelial cells (ARPE-19) were treated with the following formulations: undiluted preserved TA (TA-BA), diluted preserved TA (D-TA-BA), preservative-free TA (TA-PF), and LB-VD. First, cell tolerability was evaluated with MTT, LDH, and ATPlite assays after 1, 5, and 30 minutes of exposure to each tested formulation. Then, cells were sequentially exposed to formulations and PFD. After 24 hours of exposure to PFD, cell tolerability was evaluated through MTT and ATPlite assays.
Among the formulations tested, LB-VD showed the highest levels of cell viability, cell metabolism, and cell proliferation and induced the lowest release of LDH, whereas the TA-based formulations demonstrated a cytotoxic effect on ARPE-19 cells in vitro. After subsequent 24-hour exposure to PFD, a greater reduction of cell viability was noted for all the formulations; however, this reduction was not significant only for the combination LB-VD-PFD, which was the best tolerated condition.
LB-VD showed a better safety profile compared with all TA-based formulations, even when used in combination with PFD.
In surgical practice, LB-VD may be preferred to TA-based formulations for vitreous staining in the light of its more favorable safety profile.
评估一种新型叶黄素为基础的玻璃体染料（LB-VD）制剂与各种曲安奈德（TA）制剂（无论是否随后暴露于全氟癸烷（PFD））在体外的安全性特征。
用人成年视网膜色素上皮细胞（ARPE-19）处理以下制剂：未经稀释的保存 TA（TA-BA）、稀释保存 TA（D-TA-BA）、无防腐剂 TA（TA-PF）和 LB-VD。首先，通过 MTT、LDH 和 ATPlite 测定法在暴露于每种测试制剂 1、5 和 30 分钟后评估细胞耐受性。然后，细胞依次暴露于制剂和 PFD。暴露于 PFD 24 小时后，通过 MTT 和 ATPlite 测定法评估细胞耐受性。
在所测试的制剂中，LB-VD 显示出最高的细胞活力、细胞代谢和细胞增殖水平，并诱导最低的 LDH 释放，而基于 TA 的制剂在体外对 ARPE-19 细胞显示出细胞毒性作用。随后暴露于 PFD 24 小时后，所有制剂的细胞活力均明显降低；然而，仅对于组合 LB-VD-PFD，这种降低并不显著，这是最耐受的条件。
LB-VD 与所有基于 TA 的制剂相比表现出更好的安全性特征，即使与 PFD 联合使用也是如此。
吕晨曦
