Pukhalskaya E C, Petrova M F, Denisova S I, Alieva T A
Neoplasma. 1975;22(1):29-37.
The antineoplastic substances isolated from Chamaenerium angustifolium, Chanerol, a macromolecular polyphenol, and its complex with polysaccharide, Chanerozan, have been studied in vitro in tests of haemagglutination, tumor cell agglutination, leucoagglutination and some others. The results obtained show Chanerol as a new phytohaemagglutinin (lectin) which has no group specificity for human erythrocytes (ABO system). As distinct from other lectins (proteins) Chanerol belongs to another chemical class (macromolecular polyphenols) and has no effect on lymphocytes in leukoagglutination and blast transformation tests. Both preparations have been shown capable of agglutinating tumor cells in vitro and revealed antitumor activity in the intravenous and intraperitoneal administration to mice with transplanted tumors. The toxicity of Chanerozan and its titre of human and mouse erythrocyte agglutination are considerably lower than those of Chanerol. Thus, judging by haemagglutination test Chanerozan behaves as a typical complex of agglutinin with concurrent sugar.
从柳兰（Chamaenerium angustifolium）中分离出的抗肿瘤物质——大分子多酚查内罗尔（Chanerol）及其与多糖的复合物查内罗赞（Chanerozan），已在体外进行了血凝、肿瘤细胞凝集、白细胞凝集等多项试验研究。所得结果表明，查内罗尔是一种对人红细胞（ABO系统）无血型特异性的新型植物血凝素（凝集素）。与其他凝集素（蛋白质）不同，查内罗尔属于另一化学类别（大分子多酚），在白细胞凝集和母细胞转化试验中对淋巴细胞无作用。两种制剂均已证明能够在体外凝集肿瘤细胞，并在给移植肿瘤的小鼠静脉和腹腔注射时显示出抗肿瘤活性。查内罗赞的毒性及其对人及小鼠红细胞的凝集效价均远低于查内罗尔。因此，从血凝试验判断，查内罗赞表现为凝集素与伴随糖的典型复合物。
