Preobrazhenskaia Iu V, Burd' V N, Voskoboev A I
Grodno State University, Ozheshko ul, 22, Grodno, 230012 Belarus.
Prikl Biokhim Mikrobiol. 2001 Jul-Aug;37(4):439-43.
A bacterial non-heme chloroperoxidase from Serratia marcescens W 250 was immobilized in calcium-alginate gel. Methods for stabilization of the immobilized enzyme were developed, and some kinetic parameters of the immobilized preparations were determined. The enzyme encapsulated into the gel granules in the presence of potassium ferricyanide followed by treatment with glutaraldehyde demonstrated the highest stability under the reaction conditions.
将粘质沙雷氏菌W 250来源的一种细菌非血红素氯过氧化物酶固定在海藻酸钙凝胶中。开发了固定化酶的稳定化方法，并测定了固定化制剂的一些动力学参数。在铁氰化钾存在下将酶包封在凝胶颗粒中，然后用戊二醛处理，该酶在反应条件下表现出最高的稳定性。
