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大麦胚苯丙氨酸tRNA的分离及色谱行为

Isolation and chromatographic behaviour of phenylalanine tRNA from barley embryos.

作者信息

Labuda D, Janowicz Z, Haertlé T, Augustyniak J

出版信息

Nucleic Acids Res. 1974 Dec;1(12):1703-12. doi: 10.1093/nar/1.12.1703.

DOI:10.1093/nar/1.12.1703
PMID:4615303
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343449/
Abstract

Two fractions of phenylalanine tRNA (tRNA(Phe) (1) and tRNA(Phe) (2)) were purified by BD-cellulose and RPC-5 chromatography of crude tRNA isolated from barley embryos. Successive RPC-5 rechromatography runs of tRNA(Phe) (2) showed its conversion into more stable tRNA(Phe) (1), suggesting that the two fractions have essentially the same primary structure. Both tRNA(Phe) (1) and tRNA(Phe) (2) had about the same acceptor activity, but tRNA(Phe) (2) was aminoacylated much faster than tRNA(Phe) (1). RPC-5 chromatography of crude aminoacylated tRNA showed higher contents of phe-tRNA(Phe) (2) than of phe-tRNA(Phe) (1) but the ratio of these two fractions estimated by relative fluorescence intensity was about 1. Fluorescence spectra of tRNA(Phe) from barley embryos suggest that it contains Y base similar to Y(w) from wheat tRNA(Phe).

摘要

通过对从大麦胚中分离得到的粗制tRNA进行BD-纤维素和RPC-5层析，纯化出了苯丙氨酸tRNA的两个组分（tRNA(Phe) (1)和tRNA(Phe) (2)）。对tRNA(Phe) (2)进行连续的RPC-5再层析表明它转化为了更稳定的tRNA(Phe) (1)，这表明这两个组分具有基本相同的一级结构。tRNA(Phe) (1)和tRNA(Phe) (2)具有大致相同的接受活性，但tRNA(Phe) (2)的氨酰化速度比tRNA(Phe) (1)快得多。对粗制氨酰化tRNA进行RPC-5层析显示，phe-tRNA(Phe) (2)的含量高于phe-tRNA(Phe) (1)，但通过相对荧光强度估算的这两个组分的比例约为1。大麦胚中tRNA(Phe)的荧光光谱表明它含有与小麦tRNA(Phe)中的Y(w)类似的Y碱基。

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本文引用的文献

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The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.在苯甲酰化二乙氨基乙基纤维素上分离可溶性核糖核酸。
Biochemistry. 1967 Oct;6(10):3043-56. doi: 10.1021/bi00862a011.
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Studies on polynucleotides. LXXXII. Yeast phenylalanine transfer ribonucleic acid: partial digestion with ribonuclease T-1 and derivation of the total primary structure.多核苷酸研究。八十二。酵母苯丙氨酸转移核糖核酸：核糖核酸酶T-1的部分消化及完整一级结构的推导
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Evidence for the absence of the terminal adenine nucleotide at the amino acid-acceptor end of transfer ribonucleic acid in non-lactating bovine mammary gland and its inhibitory effect on the aminoacylation of rat liver transfer ribonucleic acid.非泌乳期奶牛乳腺中转运核糖核酸氨基酸受体末端不存在末端腺嘌呤核苷酸的证据及其对大鼠肝脏转运核糖核酸氨酰化的抑制作用。
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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
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