Roszman T L
Proc Soc Exp Biol Med. 1975 Jun;149(2):407-12. doi: 10.3181/00379727-149-38817.
The effect of various concentrations of concanavalin A (Con A) on the in vitro secondary antibody response of rabbit lymph node and spleen cells to sheep red blood cells (SRBC) was studied. Complete suppression of the IgM plaque-forming cell (PFC) response of both lymph node and spleen cultures was observed when 10 mug/ml of Con A was added at the time of initiation of the cultures whereas only partial suppression was observed when 1 mug/ml of Con A was added. Moreover, marked suppression of the immune responses of both spleen and lymph node cultures was observed when 10 mug/ml of Con A was added at 24 hr after antigenic challenge and to a lesser extent when added at 48 hr. Suppression of the IgM PFC response was also detected when spleen cultures were exposed to 10 mug/ml of Con A for as little as 2 hr after antigenic challenge. However, substantial increases in DNA synthesis were observed only in those cultures which were in contact with Con A for at least 24 hr. Finally evidence is presented that the Con A-induced suppression is mediated by a soluble substance(s).
研究了不同浓度的伴刀豆球蛋白A（Con A）对兔淋巴结和脾细胞体外针对绵羊红细胞（SRBC）的二次抗体应答的影响。在培养开始时加入10μg/ml的Con A，观察到淋巴结和脾细胞培养物中IgM空斑形成细胞（PFC）应答完全受到抑制，而加入1μg/ml的Con A时仅观察到部分抑制。此外，在抗原攻击后24小时加入10μg/ml的Con A时，观察到脾和淋巴结培养物的免疫应答受到显著抑制，在48小时加入时抑制程度较小。当脾细胞培养物在抗原攻击后暴露于10μg/ml的Con A仅2小时，也检测到IgM PFC应答受到抑制。然而，仅在与Con A接触至少24小时的那些培养物中观察到DNA合成有显著增加。最后提出证据表明Con A诱导的抑制是由一种可溶性物质介导的。
