Liu S, Luo H, Li N, Liu Z, Zheng W
Institute of Environmental Chemistry, Southwest China Normal University, Congqing.
Anal Chem. 2001 Aug 15;73(16):3907-14. doi: 10.1021/ac001454h.
In a near-neutral medium, a combination of heparin with some basic diphenyl naphthylmethane dyes such as victoria blue 4R (VB4R), victoria blue B (VBB), or night blue (NB) can result in a significant enhancement of resonance. Rayleigh scattering (RRS) and their maximum scattering wavelengths lambdamax appear at 523, 534, and 540 nm for VB4R, VBB, and NB, respectively. The characteristics of RRS spectra of the heparin-dye complexes, the influencing factors, and the optimum conditions of these reactions have been investigated. The RRS intensity is directly proportional to the concentration of heparin in the range of approximately 0 to 0.4 microg/mL for all systems. The methods exhibit high sensitivities, and the detection limits for heparin are 3.35 ng/mL for the VB4R system, 6.62 ng/ mL for the VBB system, and 6.29 ng/mL for the NB system. Because the VB4R system is the most sensitive, it was taken as an example to study the selectivity of the method. A new method for the determination of trace amounts of heparin based on RRS technique has been developed. Moreover, the enhancement reasons of RRS and the relationship between RRS spectral characteristics of the heparin-dye complex and its absorption spectra have been primarily discussed.
在近中性介质中，肝素与一些碱性二苯基萘基甲烷染料如维多利亚蓝4R（VB4R）、维多利亚蓝B（VBB）或夜蓝（NB）结合可导致共振显著增强。对于VB4R、VBB和NB，瑞利散射（RRS）及其最大散射波长λmax分别出现在523、534和540nm处。研究了肝素 - 染料复合物的RRS光谱特征、影响因素及这些反应的最佳条件。在所有体系中，RRS强度在约0至0.4μg/mL范围内与肝素浓度成正比。这些方法具有高灵敏度，VB4R体系中肝素的检测限为3.35ng/mL，VBB体系为6.62ng/mL，NB体系为6.29ng/mL。由于VB4R体系最灵敏，以其为例研究了该方法的选择性。建立了一种基于RRS技术测定痕量肝素的新方法。此外，初步探讨了RRS增强的原因以及肝素 - 染料复合物的RRS光谱特征与其吸收光谱之间的关系。
