Lin G, Palmer R E, Pister K S, Roos K P
Standard MEMS Incorporated, Rancho Palos Verdes, CA 90275, USA.
IEEE Trans Biomed Eng. 2001 Sep;48(9):996-1006. doi: 10.1109/10.942589.
A fully submersible force transducer system for use with isolated heart cells has been implemented using microelectromechanical systems (MEMS) technology. By using integrated circuit fabrication techniques to make mechanical as well as electrical components, the entire low-mass transducer is only a few cubic millimeters in size and is of higher fidelity (approximately 100 nN and 13.3 kHz in solution) than previously available. When chemically activated, demembranated single cells attached to the device contract and slightly deform a strain gauge whose signal is converted to an amplified electrical output. When integrated with a video microscope, the system is capable of optical determination of contractile protein striation periodicity and simultaneous measurement of heart cell forces in the 100-nN to 50-microN range. The average measured maximal force was Fmax = 5.77 +/- 2.38 microN. Normalizing for the cell's cross-sectional area, Fmax/area was 14.7 +/- 7.7 mN/mm2. Oscillatory stiffness data at frequencies up to 1 kHz has also been recorded from relaxed and contracted cells. This novel MEMS force transducer system permits higher fidelity measurements from cardiac myocytes than available from standard macro-sized transducers.
一种用于分离心脏细胞的全潜水式力传感器系统已采用微机电系统（MEMS）技术实现。通过使用集成电路制造技术来制造机械和电气部件，整个低质量传感器的尺寸仅为几立方毫米，并且比以前的产品具有更高的保真度（在溶液中约为100 nN和13.3 kHz）。当化学激活时，附着在该装置上的去膜单细胞收缩并使应变仪轻微变形，其信号被转换为放大的电输出。当与视频显微镜集成时，该系统能够光学测定收缩蛋白条纹的周期性，并同时测量100 nN至50 μN范围内的心脏细胞力。测得的平均最大力为Fmax = 5.77 +/- 2.38 μN。以细胞横截面积进行归一化后，Fmax/面积为14.7 +/- 7.7 mN/mm2。还记录了松弛和收缩细胞在高达1 kHz频率下的振荡刚度数据。这种新型MEMS力传感器系统允许从心肌细胞进行比标准宏观尺寸传感器更高保真度的测量。
