Bazan Carlos, Barba David Torres, Hawkins Trevor, Nguyen Hung, Anderson Samantha, Vazquez-Hidalgo Esteban, Lemus Rosa, Moore J'Terrell, Mitchell Jeremy, Martinez Johanna, Moore Delnita, Larsen Jessica, Paolini Paul
Computational Science Research Center Rees-Stealy Research Foundation Laboratory, San Diego State University, 5500 Campanile Drive, San Diego, CA, 92182-1245, USA.
Biophys Rev. 2012 Sep;4(3):231-243. doi: 10.1007/s12551-012-0082-y. Epub 2012 Sep 1.
The use of enzymatically isolated cardiac myocytes is ubiquitous in modern cardiovascular research. Parallels established between cardiomyocyte shortening responses and those of intact tissue make the cardiomyocyte an invaluable experimental model of cardiac function. Much of our understanding regarding the fundamental processes underlying heart function is owed to our increasing capabilities in single-cell stimulation and direct or indirect observation, as well as quantitative analysis of such cells. Of the many important mechanisms and functions that can be readily assessed in cardiomyocytes at all stages of development, contractility is the most representative and one of the most revealing. The purpose of this review is to provide a survey of various methodological approaches in the literature used to assess adult and neonatal cardiomyocyte contractility. The various methods employed to evaluate the contractile behavior of enzymatically isolated mammalian cardiac myocytes can be conveniently divided into two general categories-those employing optical (image)-based systems and those that use transducer-based technologies. This survey is by no means complete, but we have made an effort to include the most popular methods in terms of reliability and accessibility. These techniques are in constant evolution and hold great promise for the next generation of breakthrough studies in cell biology for the prevention, treatment, and cure of cardiovascular diseases.
在现代心血管研究中，酶解法分离的心肌细胞应用广泛。心肌细胞缩短反应与完整组织的缩短反应之间建立的相似性，使心肌细胞成为心脏功能的重要实验模型。我们对心脏功能基本过程的许多理解，都归功于我们在单细胞刺激、直接或间接观察以及此类细胞定量分析方面能力的不断提高。在心肌细胞发育的各个阶段都能轻易评估的众多重要机制和功能中，收缩性是最具代表性且最具揭示性的之一。本综述的目的是对文献中用于评估成年和新生心肌细胞收缩性的各种方法进行概述。用于评估酶解法分离的哺乳动物心肌细胞收缩行为的各种方法可方便地分为两大类——基于光学（图像）系统的方法和使用基于换能器技术的方法。本概述绝非完整，但我们已努力纳入在可靠性和可及性方面最常用的方法。这些技术不断发展，有望为预防、治疗和治愈心血管疾病的细胞生物学下一代突破性研究带来巨大希望。
