Lee Y T, Miller L D, Gubin A N, Makhlouf F, Wojda U, Barrett A J, Liu E T, Miller J L
Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Blood. 2001 Sep 15;98(6):1914-21. doi: 10.1182/blood.v98.6.1914.
Because abnormal erythroid differentiation is the most common manifestation of the myelodysplastic syndromes (MDS), it was hypothesized that erythroid gene expression may be used to illustrate myelodysplastic transcription patterns. Ten normal bone marrow aspirates (NBM) were first analyzed using an erythroid-focused cDNA array to define steady-state transcription levels. Proliferation and differentiation gene subsets were identified by statistically significant differences between NBM and erythroleukemia gene expression. Next, cDNAs from 5 separate MDS aspirates were studied: refractory anemia, refractory anemia with ringed sideroblasts, refractory anemia with excess blasts, refractory anemia with excess blasts in transformation (RAEB-T), and RAEB-T/secondary MDS. A distinct pattern of significantly increased proliferation-associated and reduced differentiation-associated gene activity was established for MDS.
由于异常红系分化是骨髓增生异常综合征（MDS）最常见的表现，因此有人推测红系基因表达可用于阐释骨髓增生异常的转录模式。首先使用以红系为重点的cDNA阵列分析了10份正常骨髓穿刺物（NBM），以确定稳态转录水平。通过NBM与红白血病基因表达之间的统计学显著差异鉴定出增殖和分化基因亚群。接下来，研究了来自5份不同MDS穿刺物的cDNA：难治性贫血、环形铁粒幼细胞性难治性贫血、原始细胞增多的难治性贫血、转化中的原始细胞增多的难治性贫血（RAEB-T）以及RAEB-T/继发性MDS。为MDS建立了增殖相关基因活性显著增加和分化相关基因活性降低的独特模式。
