人坏死胰腺组织中游离脂肪酸和甘油三酯组分的构成
Composition of free fatty acid and triglyceride fractions in human necrotic pancreatic tissue.
作者信息
Panek J, Sztefko K, Drozdz W
机构信息
2nd Department of General Surgery, Collegium Medicum, Jagiellonian University, Cracow, Poland.
出版信息
Med Sci Monit. 2001 Sep-Oct;7(5):894-8.
BACKGROUND
Lipolytic enzymes, such as lipase, phospholipase A(2), lipoprotein lipase, and hormone-sensitive adipocyte lipase are probably implicated in the pathogenesis of acute pancreatitis. The increased activity of these enzymes can cause specific changes in fatty acid composition, both to free fatty acid (FFA) and triglyceride (TG) fractions in necrotically changed pancreatic tissue.
MATERIAL AND METHODS
Specimens of necrotically changed pancreatic tissue taken intraoperatively from 13 patients operated for serious necrotic acute pancreatitis were analysed. The Fölsch extraction method and separation of lipids by thin-layer chromatography was used, and the final fatty acid composition was determined after methylation by gas liquid chromatography.
RESULTS
The changes in the FFA fraction are more definite than those for the triglyceride fraction and refer mostly to the increased percentage distribution of unsaturated fatty acids.
CONCLUSIONS
These studies lead to the hypothesis that fatty acids released in the process of lipolysis play a key role in the pathogenesis of acute pancreatitis.
背景
脂解酶，如脂肪酶、磷脂酶A(2)、脂蛋白脂肪酶和激素敏感性脂肪细胞脂肪酶，可能与急性胰腺炎的发病机制有关。这些酶活性的增加可导致坏死性改变的胰腺组织中游离脂肪酸(FFA)和甘油三酯(TG)部分的脂肪酸组成发生特定变化。
材料与方法
分析了13例因严重坏死性急性胰腺炎接受手术治疗的患者术中获取的坏死性改变的胰腺组织标本。采用Fölsch提取法和薄层色谱法分离脂质，通过气相色谱法甲基化后测定最终脂肪酸组成。
结果
FFA部分的变化比甘油三酯部分更明确，主要表现为不饱和脂肪酸的百分比分布增加。
结论
这些研究得出一个假设，即脂解过程中释放的脂肪酸在急性胰腺炎的发病机制中起关键作用。
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