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[用于血清脂蛋白组分琼脂糖电泳分离的简单、快速且经济的方法]

[Simple rapid and economical procedure for agarose electrophoretic separation of serum lipoprotein fractions].

作者信息

Menegozzo A, Guidi M T, De Cecchi S, Lippi U

出版信息

Quad Sclavo Diagn. 1975 Mar;11(1):147-53.

PMID:1153695
Abstract

A detailed description is given of a rapid, simple and economic technique for the electrophoretic migration on agarose gel of serum lipoproteins. The albuminated agarose is spread hot on pieces of normal radiological plates even if already used. The reservoir for the serum is obtained by putting in the agarose while still hot a series of small metal bars. The moulds are removed with a small magnet as soon as the gel is formed. Plates prepared for 6-7 lipidograms can comfortably be kept for as long as 20-30 days. For stored plates ready for use, the analytic procedure does not require more than 40-45 minutes. The results can be considered altogether satisfactory.

摘要

本文详细描述了一种用于血清脂蛋白在琼脂糖凝胶上进行电泳迁移的快速、简单且经济的技术。即使是已经用过的普通放射学平板,也可将含白蛋白的琼脂糖趁热铺在上面。通过在琼脂糖仍热的时候放入一系列小金属棒来形成血清储存槽。凝胶一旦形成,就用小磁铁将模具取出。为6 - 7次血脂图准备的平板可以舒适地保存长达20 - 30天。对于已准备好使用的储存平板,分析过程所需时间不超过40 - 45分钟。结果可认为完全令人满意。

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