López G-Coviella I, Agut J, Ortiz J A, Wurtman R J
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge.
J Nutr Biochem. 1992 Jun;3(6):313-5. doi: 10.1016/0955-2863(92)90039-l.
Cytidine, as cytidine 5'-diphosphate choline (CDP-choline), is important for the synthesis of phosphatidylcholine in cell membranes. To investigate whether exogenous CDP-choline could affect brain phospholipid composition, we supplemented the diet of mice with this drug (500 mg/kg/day) for 27 months in 3-month-old mice and for 90, 42, and 3 days in 12-month-old mice, and measured their levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and the content of phosphatidylinositol plus phosphatidic acid in the cerebral cortex. After 27 months of treatment, PC and PE increased significantly by 19% (P < 0.05) and by 20% (P < 0.01), respectively. PS levels increased by 18% (not statistically significant). Similar elevations in PC and PE levels were obtained when older mice were treated for only 3 months (P < 0.05). No changes were observed with shorter treatment periods. These results suggest that chronic administration of CDP-choline can have effects on brain phospholipid composition that may underlie its reported utility in various neurologic disorders.
胞苷，作为胞苷5'-二磷酸胆碱（CDP-胆碱），对于细胞膜中磷脂酰胆碱的合成很重要。为了研究外源性CDP-胆碱是否会影响脑磷脂组成，我们在3月龄小鼠中用这种药物（500 mg/kg/天）补充饮食27个月，在12月龄小鼠中分别补充90天、42天和3天，并测量它们大脑皮质中磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）的水平以及磷脂酰肌醇加磷脂酸的含量。治疗27个月后，PC和PE分别显著增加了19%（P < 0.05）和20%（P < 0.01）。PS水平增加了18%（无统计学意义）。当老年小鼠仅治疗3个月时，也获得了类似的PC和PE水平升高（P < 0.05）。较短治疗期未观察到变化。这些结果表明，长期给予CDP-胆碱可对脑磷脂组成产生影响，这可能是其在各种神经系统疾病中所报道效用的基础。
