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生长温度对荚膜甲基球菌(巴斯德菌株)甲基甾醇和磷脂脂肪酸组成的影响

The effects of growth temperature on the methyl sterol and phospholipid fatty acid composition of Methylococcus capsulatus (Bath).

作者信息

Jahnke L L

机构信息

NASA-Ames Research Center, Moffett Field, CA.

出版信息

FEMS Microbiol Lett. 1992 Jun 15;72(3):209-12. doi: 10.1111/j.1574-6968.1992.tb05099.x.

DOI:10.1111/j.1574-6968.1992.tb05099.x
PMID:11537858
Abstract

Growth of Methylococcus capsulatus (Bath) at temperatures ranging from 30 to 50 degrees C resulted in changes to the whole cell lipid constituents. As temperature was lowered, the overall proportion of hexadecenoic acid (C16:1) increased, and the relative proportions of the delta 9, delta 10 and delta 11 C16:1 double bond positional isomers changed. Methyl sterol content also increased as the growth temperature was lowered. The highest amounts of methyl sterol were found in 30 degrees C cells and the lowest in 50 degrees C cells (sterol-phospholipid ratios of 0.077 and 0.013, respectively). The data are consistent with a membrane modulating role for the sterol produced by this prokaryotic organism.

摘要

甲基球菌(巴斯德菌株)在30至50摄氏度的温度范围内生长,导致全细胞脂质成分发生变化。随着温度降低,十六碳烯酸(C16:1)的总体比例增加,并且δ9、δ10和δ11 C16:1双键位置异构体的相对比例发生变化。甲基甾醇含量也随着生长温度的降低而增加。在30摄氏度的细胞中发现甲基甾醇含量最高,在50摄氏度的细胞中最低(甾醇与磷脂的比例分别为0.077和0.013)。这些数据与这种原核生物产生的甾醇的膜调节作用一致。

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