Sobota J T, Gruber C A, Gilbertson T J, Wilson J
Am J Hematol. 1977;2(2):133-43. doi: 10.1002/ajh.2830020205.
Prostaglandin E2 (PGE2), at concentration larger than or equal to 5 x 10(4) ng/ml, induced discocyte leads to echinocyte transformation of saline-suspended hemoglobin (Hb) AA and SS erythrocytes. This erythrocyte transformation is concentration-dependent and is reversible at room temperature after 90-120 min. The Hb SS erythrocytes treated with PGE2 did not exhibit accelerated sickling or increased formation of sickled echinocytes. Erythrocytes suspended in autologous plasma treated with PGE2, 2 X 10(6) ng/ml, did not exhibit echinocytic transformation probably because of drug binding to the plasma proteins. Other in vitro studies showed that PGE2 of concentrations of 10-500 ng/ml had no adverse effects on intact, plasma-suspended Hb SS erythrocytes. These Hb SS erythrocytes were examined for changes in morphology, potassium and calcium flux, and blood viscosity under oxygenated and hypoxic conditions.
前列腺素E2（PGE2），浓度大于或等于5×10⁴ ng/ml时，可诱导盘状红细胞导致盐水悬浮的血红蛋白（Hb）AA和SS红细胞转变为棘状红细胞。这种红细胞转变具有浓度依赖性，并且在室温下90 - 120分钟后是可逆的。用PGE2处理的Hb SS红细胞未表现出加速镰变或镰状棘状红细胞形成增加。悬浮于用2×10⁶ ng/ml PGE2处理的自体血浆中的红细胞未表现出棘状红细胞转变，可能是因为药物与血浆蛋白结合。其他体外研究表明，浓度为10 - 500 ng/ml的PGE2对完整的、血浆悬浮的Hb SS红细胞没有不良影响。在有氧和缺氧条件下，对这些Hb SS红细胞进行了形态、钾和钙通量以及血液粘度变化的检查。
