三体胸节SET结构域中的同源异型突变会阻碍组蛋白结合。
A homeotic mutation in the trithorax SET domain impedes histone binding.
作者信息
Katsani K R, Arredondo J J, Kal A J, Verrijzer C P
机构信息
Department of Molecular and Cell Biology, MGC Center for Biomedical Genetics, Leiden University Medical Center, 2300 RA Leiden, The Netherlands.
出版信息
Genes Dev. 2001 Sep 1;15(17):2197-202. doi: 10.1101/gad.201901.
Abstract
Trithorax (TRX) is a Drosophila SET domain protein that is required for the correct expression of homeotic genes. Here, we show that the TRX SET domain efficiently binds to core histones and nucleosomes. The primary target for the SET domain is histone H3 and binding requires the N-terminal histone tails. The previously described trx(Z11) mutation changes a strictly conserved glycine in the SET domain to serine and causes homeotic transformations in the fly. We found that this mutation selectively interferes with histone binding, suggesting that histones represent a critical target during developmental gene regulation by TRX.
摘要
三体胸节蛋白（TRX）是一种果蝇SET结构域蛋白，对同源异型基因的正确表达至关重要。在此，我们表明TRX SET结构域能有效结合核心组蛋白和核小体。SET结构域的主要靶点是组蛋白H3，且结合需要组蛋白的N端尾巴。先前描述的trx(Z11)突变将SET结构域中一个严格保守的甘氨酸变为丝氨酸，并在果蝇中导致同源异型转化。我们发现该突变选择性地干扰组蛋白结合，这表明组蛋白是TRX在发育基因调控过程中的关键靶点。
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