VanBuren Vincent, Piao Yulan, Dudekula Dawood B, Qian Yong, Carter Mark G, Martin Patrick R, Stagg Carole A, Bassey Uwem C, Aiba Kazuhiro, Hamatani Toshio, Kargul George J, Luo Amber G, Kelso Janet, Hide Winston, Ko Minoru S H
Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging (NIA), National Institutes of Health, Baltimore, Maryland, 20892, USA.
Genome Res. 2002 Dec;12(12):1999-2003. doi: 10.1101/gr.633802.
A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ~120,000 cDNA clones, which were initially re-arrayed into a set of ~11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.
一组7407个cDNA克隆（NIA小鼠7.4K）由主要从早期小鼠胚胎构建的20多个cDNA文库组装而成，包括几个干细胞文库。该克隆集由包含约120,000个cDNA克隆的胚胎和新生器官文库组装而成，这些克隆最初被重新排列成一组约11,000个独特的cDNA克隆。从该组的架子上构建了一组管子，以防止在所有进一步的重新排列中受到污染和潜在的操作失误。对该组（11K）的序列进行了进一步的质量和克隆身份分析，具有验证身份的高质量克隆被重新排列到最终组（7.4K）中。该数据集可免费获取，并建立了一个相应的数据库，为那些具有已知身份或同源性的克隆提供全面注释，并且已经通过一个广泛的网站提供，该网站包括许多指向外部数据库和分析服务器的链接。
