Boyd D, Peters G A, Cloeckaert A, Boumedine K S, Chaslus-Dancla E, Imberechts H, Mulvey M R
National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba R3E 3R2, Canada.
J Bacteriol. 2001 Oct;183(19):5725-32. doi: 10.1128/JB.183.19.5725-5732.2001.
This study describes the characterization of the recently described Salmonella genomic island 1 (SGI1) (D. A. Boyd, G. A. Peters, L.-K. Ng, and M. R. Mulvey, FEMS Microbiol. Lett. 189:285-291, 2000), which harbors the genes associated with the ACSSuT phenotype in a Canadian isolate of Salmonella enterica serovar Typhimurium DT104. A 43-kb region has been completely sequenced and found to contain 44 predicted open reading frames (ORFs) which comprised approximately 87% of the total sequence. Fifteen ORFs did not show any significant homology to known gene sequences. A number of ORFs show significant homology to plasmid-related genes, suggesting, at least in part, a plasmid origin for the SGI1, although some with homology to phage-related genes were identified. The SGI1 was identified in a number of multidrug-resistant DT120 and S. enterica serovar Agona strains with similar antibiotic-resistant phenotypes. The G+C content suggests a potential mosaic structure for the SGI1. Emergence of the SGI1 in serovar Agona strains is discussed.
本研究描述了最近发现的沙门氏菌基因组岛1（SGI1）（D. A. Boyd、G. A. Peters、L.-K. Ng和M. R. Mulvey，《FEMS微生物学快报》189:285 - 291，2000年）的特征，该基因组岛在一株加拿大肠炎沙门氏菌鼠伤寒血清型DT104分离株中携带与ACSSuT表型相关的基因。一个43 kb的区域已被完全测序，发现包含44个预测的开放阅读框（ORF），约占总序列的87%。15个ORF与已知基因序列没有任何显著同源性。一些ORF与质粒相关基因显示出显著同源性，这至少部分表明SGI1起源于质粒，尽管也鉴定出了一些与噬菌体相关基因具有同源性的ORF。在一些具有相似抗生素耐药表型的多重耐药DT120和肠炎沙门氏菌阿贡纳血清型菌株中鉴定出了SGI1。G + C含量表明SGI1可能具有镶嵌结构。文中还讨论了SGI1在阿贡纳血清型菌株中的出现情况。
