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乳源丙酸杆菌16S - 23S和23S - 5S rRNA基因间隔区的特征分析及其通过PCR用种特异性引物进行鉴定

Characterization of the 16S-23S and 23S-5S rRNA intergenic spacer regions of dairy propionibacteria and their identification with species-specific primers by PCR.

作者信息

Tilsala-Timisjärvi A, Alatossava T

机构信息

Department of Biology, University of Oulu, Finland.

出版信息

Int J Food Microbiol. 2001 Aug 15;68(1-2):45-52. doi: 10.1016/s0168-1605(01)00462-7.

DOI:10.1016/s0168-1605(01)00462-7
PMID:11545219
Abstract

In this study, the 16S-23S and 23S-5S rRNA intergenic spacer region sequences of Propionibacterium acidipropionici, P. freudenreichii ssp. freudenreichii and ssp. shermanii, P. jensenii and P. thoenii were determined. The sequences were shown to vary greatly between the species. Specific primer pairs were derived from the 16S-23S rRNA spacer sequences and used for the identification of the species by PCR.

摘要

在本研究中，测定了丙酸丙酸杆菌、费氏丙酸杆菌弗氏亚种和谢氏亚种、詹氏丙酸杆菌和托氏丙酸杆菌的16S - 23S以及23S - 5S rRNA基因间隔区序列。结果表明，这些序列在不同物种间差异很大。从16S - 23S rRNA间隔序列中推导得到特异性引物对，并用于通过PCR对这些物种进行鉴定。

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